Immunogold Labelling of Fatty Acyl Chains

[en] For the first time, antibodies against a hydrophobic hapten have been used for immunogold labelling of a lipid antigen (BSA-C18:1 conjugate) coated on polystyrene. The labelling was visualised either directly in transmission electron microscopy or in light microscopy after silver enhancement. Good recognition of the fatty acyl chain was obtained even after treatment of the antigen coat with various cross-linking fixatives used for electron microscopy, i.e. formaldehyde, glutaraldehyde and osmium tetroxide.

Disciplines :

Anatomy (cytology, histology, embryology...) & physiology

Author, co-author :

Compère, Philippe ; Université de Liège - ULiège > Département des sciences et gestion de l'environnement > Département des sciences et gestion de l'environnement

Maneta-Peyret, Lilly

Goffinet, Gerhard ; Université de Liège - ULiège > Services généraux (Faculté des sciences) > Relations académiques et scientifiques (Sciences)

Cassagne, Claude

Language :

English

Title :

Immunogold Labelling of Fatty Acyl Chains

Publication date :

26 April 1995

Journal title :

Journal of Immunological Methods

ISSN :

0022-1759

eISSN :

1872-7905

Publisher :

Elsevier Science

Volume :

181

Issue :

Pages :

201-9

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 24 November 2010

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Bibliography

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