Reference : Quantitative measurement of metal ions concentration of proteins separated by electro...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Physical, chemical, mathematical & earth Sciences : Physics
http://hdl.handle.net/2268/76709
Quantitative measurement of metal ions concentration of proteins separated by electrophoresis
English
Weber, Georges [Université de Liège - ULiège > > Centre européen en archéométrie - Physique nucléaire, atomique et spectroscopie >]
Strivay, David mailto [Université de Liège - ULiège > Département de physique > Physique nucléaire, atomique et spectroscopie - Centre européen en archéométrie >]
Menendez, M. [ > > ]
Schoefs, B. [ > > ]
Bertrand, M. [ > > ]
1996
International Journal of PIXE
World Scientific Publishing Company
1-2
215-225
Yes (verified by ORBi)
International
0129-0835
[en] This communication is devoted to nature determination and quantification by PIXE of metals contained in proteins after their separation by PolyAcrylamide Gel Electrophoresis (PAGE). After the electrophoresis, the gel is dried and each track is scanned with a 2.5 MeV proton beam which triggers metal X-ray fluorescence and then, allows to determine the type of metals contained in an electrophoretic band. For quantitative determination of the amount of the metal contained inside the band, the characteristic X-ray peak area is compared with those obtained with polyacrylamide gels doped with the same metal. The normalization has been achieved by using RBS measurements on the gel itself.
The procedure presented seems to be a very useful multielementary method for the metal content analysis and for the determination of the metal amounts inside proteins after their separation by electrophoresis. Furthermore it allows to check if metals remain bound to proteins.
http://hdl.handle.net/2268/76709

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