[en] Late blight of potato, caused by Phytophthora infestans was studied by using a resistant clone of potato on one side and a susceptible clone on the other side. A gene coding putatively for an alpha-galactosidase has been isolated by mRNA reverse transcription polymerase chain reaction differential display and was shown to be differentially expressed between the resistant and the susceptible clone. alpha-Galactosidases catalyse the hydrolysis of alpha-1,6 linked alpha-galactose residues from oligosaccharides and it could be shown in the present work that raffinose content decreases at 30 h after infection by P. infestans in the resistant clone.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Evers, Danièle
Ghislain, Marc
Hoffmann, Lucien
Hausman, Jean-François
Dommes, Jacques ; Université de Liège - ULiège > Département des sciences de la vie > Biologie moléculaire et biotechnologie végétales
Language :
English
Title :
A late blight resistant potato plant overexpresses a gene coding for alpha-galactosidase upon infection by Phytophthora infestans
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