Article (Scientific journals)
Decalcifying odontocete ears following a routine protocol with RDO (R)
Morell, M.; Degollada, E.; Alonso, J. M. et al.
2009In Journal of Experimental Marine Biology and Ecology, 376 (2), p. 55-58
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Abstract :
[en] The study of the organ of Corti is essential to assess the impact of underwater noise on cetaceans. While classical histology techniques (including EDTA decalcification) have been previously considered, the process is time consuming. Independently from the histological technique, one of the challenging steps after extraction and fixation of the samples is to decalcify the bone envelope to access the cochlea without damaging the soft tissues. Here, we propose to use a fast commercial decalcifier (RDO (R)). 93 ears from 11 different odontocetes species stranded in the Mediterranean, Spanish North Atlantic and North Sea were used to precisely determine the decalcification time. Depending on the tympanic-periotic volume of the species, the decalcification time ranged from several hours to a few days, allowing a subsequently faster observation of the cochlear structures through routine microscope techniques. (C) 2009 Elsevier B.V. All rights reserved.
Disciplines :
Aquatic sciences & oceanology
Author, co-author :
Morell, M.
Degollada, E.
Alonso, J. M.
Jauniaux, Thierry ;  Université de Liège - ULiège > Département de morphologie et pathologie > Département de morphologie et pathologie
Andre, Marie-Elizabeth ;  Centre Hospitalier Universitaire de Liège - CHU > Chimie médicale
Language :
English
Title :
Decalcifying odontocete ears following a routine protocol with RDO (R)
Publication date :
2009
Journal title :
Journal of Experimental Marine Biology and Ecology
ISSN :
0022-0981
Publisher :
Elsevier Science, Amsterdam, Netherlands
Volume :
376
Issue :
2
Pages :
55-58
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 22 November 2010

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