Article (Scientific journals)
Superinfection prevents recombination of the alphaherpesvirus bovine herpesvirus 1
Meurens, F.; Schynts, F.; Keil, G. M. et al.
2004In Journal of Virology, 78 (8), p. 3872-3879
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Abstract :
[en] Homologous recombination between strains of the same alphaherpesvirus species occurs frequently both in vitro and in vivo. This process has been described between strains of herpes simplex virus type 1, herpes simplex virus type 2, pseudorabies virus, feline herpesvirus 1, varicella-zoster virus, and bovine herpesvirus 1 (BoHV-1). In vivo, the rise of recombinant viruses can be modulated by different factors, such as the dose of the inoculated viruses, the distance between inoculation sites, the time interval between inoculation of the first and the second virus, and the genes in which the mutations are located. The effect of the time interval between infections with two distinguishable BoHV-1 on recombination was studied in three ways: (i) recombination at the level of progeny viruses, (ii) interference induced by the first virus infection on beta-gallactosidase gene expression of a superinfecting virus, and (iii) recombination at the level of concatemeric DNA. A time interval of 2 to 8 h between two successive infections allows the establishment of a barrier, which reduces or prevents any successful superinfection needed to generate recombinant viruses. The dramatic effect of the time interval on the rise of recombinant viruses is particularly important for the risk assessment of recombination between glycoprotein E-negative marker vaccine and field strains that could threaten BoHV-1 control and eradication programs.
Disciplines :
Immunology & infectious disease
Author, co-author :
Meurens, F.
Schynts, F.
Keil, G. M.
Muylkens, Benoît ;  Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Virologie, épidémiologie et pathologie des maladies virales
Vanderplasschen, Alain ;  Université de Liège - ULiège > Immunologie et vaccinologie
Gallego, P.
Thiry, Etienne ;  Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Virologie, épidémiologie et pathologie des maladies virales
Language :
English
Title :
Superinfection prevents recombination of the alphaherpesvirus bovine herpesvirus 1
Publication date :
April 2004
Journal title :
Journal of Virology
ISSN :
0022-538X
eISSN :
1098-5514
Publisher :
Amer Soc Microbiology, Washington, United States - Washington
Volume :
78
Issue :
8
Pages :
3872-3879
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 04 March 2009

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