Article (Scientific journals)
Viral induction of Zac1b through TLR3- and IRF3-dependent pathways
Warzée, Barbara; Mesnil, Claire; Hober, D. et al.
2010In Molecular Immunology, 48 (1-3), p. 119-127
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Keywords :
Poly(I:C); Zac1; TLR3; IRF3; Virus
Abstract :
[en] Zinc finger protein regulator of apoptosis and cell cycle arrest (Zac1) is a transcription factor able to induce apoptosis or cell cycle arrest through independent pathways. In spite of the important potential functions attributed to Zac1, little is known of its physiological regulation and biological function. We discovered that variant Zac1b was expressed in murine embryonic fibroblasts (MEFs) treated with polyriboinosinic polyribocytidylic acid [poly(I:C)], a synthetic double-stranded RNA. This regulation occurred mainly through Toll-Like Receptor 3 (TLR3)- and Interferon Regulatory Factor 3 (IRF3)-dependent pathways. As TLR3 and IRF3 are central activators of antiviral immunity, we hypothesized that Zac1 may be implicated in antiviral responses. In line with this notion, we observed that Zac1b was expressed in MEFs infected with Encephalomyocarditis virus (EMCV). We also observed that Zac1-deficient MEFs were less sensitive to EMCV-induced cell death than wild-type MEFs. However, Zac1 gene inactivation had no effect on the survival of mice infected with EMCV. In conclusion, this study describes for the first time a transcriptional regulation of Zac1b, induced by synthetic dsRNA and RNA viruses, the functional significance of which remains to be further investigated.
Disciplines :
Veterinary medicine & animal health
Biochemistry, biophysics & molecular biology
Author, co-author :
Warzée, Barbara ;  Université de Liège - ULiège > Département de sciences fonctionnelles > GIGA-R : Biochimie et biologie moléculaire
Mesnil, Claire ;  Université de Liège - ULiège > Département de sciences fonctionnelles > GIGA-R : Biochimie et biologie moléculaire
Hober, D.;  University Lille2 > FacultyofMedicine,CHRULille,LaboratoryofVirology/EA3610,Lille59037,France
Caloone, D.;  University Lille2 > FacultyofMedicine,CHRULille,LaboratoryofVirology/EA3610,Lille59037,France
Pirottin, Dimitri ;  Université de Liège - ULiège > Département de sciences fonctionnelles > Département de sciences fonctionnelles
Garigliany, Mutien-Marie  ;  Université de Liège - ULiège > Département de morphologie et pathologie > Pathologie spéciale et autopsies
Desmecht, Daniel ;  Université de Liège - ULiège > Département de morphologie et pathologie > Pathologie spéciale et autopsies
Drion, Pierre ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > GIGA-R:Méth. expér.des anim. de labo et éth. en expér. anim. - GIGA-R : Services généraux de l'Université
Journot, L.;  Institut deGénomiqueFonctionnelle,Montpellier,France
Lekeux, Pierre ;  Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie
Bureau, Fabrice  ;  Université de Liège - ULiège > Département de sciences fonctionnelles > GIGA-R : Biochimie et biologie moléculaire
Desmet, Christophe   ;  Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie
 These authors have contributed equally to this work.
Language :
English
Title :
Viral induction of Zac1b through TLR3- and IRF3-dependent pathways
Publication date :
2010
Journal title :
Molecular Immunology
ISSN :
0161-5890
Publisher :
Pergamon-Elsevier Science Ltd, Oxford, United Kingdom
Volume :
48
Issue :
1-3
Pages :
119-127
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 19 November 2010

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