Cocaine and Amphetamine-Regulated-Transcript Peptide Mediation of Leptin Stimulatory Effect on the Rat Gonadotropin-Releasing Hormone Pulse Generator in Vitro

LEBRETHON, Marie-Christine; Vandersmissen, E.; Gerard, Arlette; Parent, Anne-Simone; Bourguignon, Jean-Pierre

doi:10.1046/j.1365-2826.2000.00497.x

Article (Scientific journals)

Cocaine and Amphetamine-Regulated-Transcript Peptide Mediation of Leptin Stimulatory Effect on the Rat Gonadotropin-Releasing Hormone Pulse Generator in Vitro

LEBRETHON, Marie-Christine; Vandersmissen, E.; Gerard, Arlette et al.

2000 • In Journal of Neuroendocrinology, 12 (5), p. 383-5

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/76072

DOI
10.1046/j.1365-2826.2000.00497.x

PubMed
10792575

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Keywords :

gnrh; hypothalamus

Abstract :

[en] Pulsatile gonadotropin-releasing hormone (GnRH) secretion was studied in vitro using explants of the retrochiasmatic hypothalamus from prepubertal male and female rats. Leptin caused a dose-dependent reduction of the GnRH interpulse interval in both sexes. We studied the effects of cocaine- and amphetamine-regulated transcript (CART) since this peptide was shown recently to mediate the anorectic effects of leptin in the hypothalamus. CART caused a reduction of the GnRH interpulse interval. This effect was prevented using an anti-CART antiserum which could partially overcome leptin stimulatory effects as well. Using hypothalamic explants from Zucker rats homozygous for the leptin receptor mutation ( fa/fa), GnRH pulse frequency was not affected by leptin, while a significant acceleration was caused by the CART-peptide. In conclusion, leptin involves the hypothalamic CART-peptide to stimulate the prepubertal GnRH pulse generator in vitro.

Disciplines :

Endocrinology, metabolism & nutrition

Author, co-author :

LEBRETHON, Marie-Christine ; Centre Hospitalier Universitaire de Liège - CHU > Pédiatrie

Vandersmissen, E.

Gerard, Arlette ; Centre Hospitalier Universitaire de Liège - CHU > Pédiatrie

Parent, Anne-Simone ; Université de Liège - ULiège > Département des sciences cliniques > Pédiatrie

Bourguignon, Jean-Pierre ; Université de Liège - ULiège > Département des sciences cliniques > Pédiatrie

Language :

English

Title :

Cocaine and Amphetamine-Regulated-Transcript Peptide Mediation of Leptin Stimulatory Effect on the Rat Gonadotropin-Releasing Hormone Pulse Generator in Vitro

Publication date :

May 2000

Journal title :

Journal of Neuroendocrinology

ISSN :

0953-8194

eISSN :

1365-2826

Publisher :

Wiley, Malden, United States - Massachusetts

Volume :

Issue :

Pages :

383-5

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 19 November 2010

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