Article (Scientific journals)
Inositol 1,4,5-trisphosphate 3-kinase B controls survival and prevents anergy in B cells
Marechal, Yoann; Queant, Séverine; Polizzi, Séléna et al.
2011In Immunobiology, 216, p. 103-109
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Keywords :
B cell survival; Anergy; Bim; Itpkb; Lymphocyte selection
Abstract :
[en] Inositol 1,4,5-trisphosphate 3-kinase B (or Itpkb) and inositol 1,3,4,5-tetrakisphosphate (Ins(1,3,4,5)P4), its reaction product, play an important role in the control of B lymphocyte fate and function in vivo. In order to investigate the fine mechanisms of Itpkb and Ins(1,3,4,5)P4 action in B cells, we crossed Itpkb(-/-) mice with transgenic mice expressing a 3-83mudelta B cell receptor (BCR) specific for membrane-bound MHC-I H2-K(b) and H2-K(k) molecules. On a non-deleting H2-K(d) genetic background, we show that Itpkb is important for the control of Bim protein expression and B cell survival rather than for the control of B cell development from one stage to another. Analyses of cell surface markers expression, proapoptotic Bim protein expression, in vitro survival and in vivo turnover demonstrated that BCR transgenic Itpkb(-/-) B cells exhibit an anergic phenotype with the notable exception of their enhanced antigen-induced calcium signalling. On a deleting H2-K(b) genetic background, we show that Itpkb is not essential for BCR editing or negative selection. These data establish Itpkb as an important regulator of B cell survival and anergy in vivo.
Disciplines :
Biochemistry, biophysics & molecular biology
Genetics & genetic processes
Author, co-author :
Marechal, Yoann;  Université Libre de Bruxelles - ULB > Institut de Recherches Interdisciplinaires en Biologie Humaine et Moléculaire (IRIBHM), Institut de Biologie et de Médecine Moléculaires (IBMM), Faculté de Médecine
Queant, Séverine;  Université Libre de Bruxelles - ULB > Institut de Recherches Interdisciplinaires en Biologie Humaine et Moléculaire (IRIBHM), Institut de Biologie et de Médecine Moléculaires (IBMM), Faculté de Médecine
Polizzi, Séléna;  Université Libre de Bruxelles - ULB > Institut de Recherches Interdisciplinaires en Biologie Humaine et Moléculaire (IRIBHM), Institut de Biologie et de Médecine Moléculaires (IBMM), Faculté de Médecine
Pouillon, Valérie;  Université Libre de Bruxelles - ULB > Institut de Recherches Interdisciplinaires en Biologie Humaine et Moléculaire (IRIBHM), Institut de Biologie et de Médecine Moléculaires (IBMM), Faculté de Médecine
Schurmans, Stéphane  ;  Université de Liège - ULiège > Département de sciences fonctionnelles, Biochimie métabolique vétérinaire > et GIGA-Research
Language :
English
Title :
Inositol 1,4,5-trisphosphate 3-kinase B controls survival and prevents anergy in B cells
Publication date :
2011
Journal title :
Immunobiology
ISSN :
0171-2985
Publisher :
Gustav Fischer Verlag, Stuttgart, Germany
Volume :
216
Pages :
103-109
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 16 November 2010

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