Article (Scientific journals)
The Er-Tr4 Monoclonal Antibody Recognizes Murine Thymic Epithelial Cells (Type 1) and Inhibits Their Capacity to Interact with Immature Thymocytes: Immuno-Electron Microscopic and Functional Studies
Defresne, Marie-Paule; Nabarra, B.; van Vliet, E. et al.
1994In Histochemistry, 101 (5), p. 355-63
Peer Reviewed verified by ORBi
 

Files


Full Text
DefresneMP_65_ER-TR4-monoclonal-antibody-1994.pdf
Publisher postprint (1.66 MB)
Download

All documents in ORBi are protected by a user license.

Send to



Details



Abstract :
[en] The thymic stroma is heterogeneous with regard to cellular morphology and cellular function. In this study, we employed the monoclonal antibody ER-TR4 to characterize stromal cells at the ultrastructural level. To identify the labelled cell type, we used two techniques: immunogold labelling on ultrathin frozen sections and immunoperoxidase staining on thick "vibratome" sections. ER-TR4 reacted with thymic Type 1 epithelial cells (according to our classification). A dense labelling appears in the cytoplasm of cortical cells using the two techniques. Immunogold labelling identified small cytoplasmic vesicles whereas the cytoplasm and the cell membrane seem to be labelled with the immunoperoxidase technique. ER-TR4 also identified isolated thymic nurse cells (TNC), and was observed in vitro to inhibit the capacity of some type 1 epithelial cells to establish interactions with immature thymocytes. This finding supports the hypothesis that the factor is involved in the formation of lymphoepithelial interactions within thymic nurse cells, and thus in the relations that immature thymocytes establish with the thymic microenvironment.
Disciplines :
Anatomy (cytology, histology, embryology...) & physiology
Author, co-author :
Defresne, Marie-Paule ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie - Cytologie
Nabarra, B.
van Vliet, E.
Willemsen, R.
van Dongen, H.
van Ewijk, W.
Language :
English
Title :
The Er-Tr4 Monoclonal Antibody Recognizes Murine Thymic Epithelial Cells (Type 1) and Inhibits Their Capacity to Interact with Immature Thymocytes: Immuno-Electron Microscopic and Functional Studies
Publication date :
June 1994
Journal title :
Histochemistry
ISSN :
0301-5564
Publisher :
Springer, Germany
Volume :
101
Issue :
5
Pages :
355-63
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 15 November 2010

Statistics


Number of views
22 (5 by ULiège)
Number of downloads
8 (0 by ULiège)

Scopus citations®
 
7
Scopus citations®
without self-citations
7
OpenCitations
 
7

Bibliography


Similar publications



Contact ORBi