This is an electronic version (Author’s preprint) of the article published in American Journal of Reproductive Immunology, 2010 Sept 64(3):218-24 The original published version is available at:http://onlinelibrary.wiley.com/doi/10.1111/j.1600-0897.2010.00859.x/pdf
[en] Citation Tskitishvili E, Sharentuya N, Tsubouchi H, Kinugasa-Taniguchi Y, Kanagawa T, Shimoya K, Tomimatsu T, Kimura T. Maternal blood serum and plasma human tumor-associated antigen RCAS1 during the course of uncomplicated pregnancies: a prospective study. Am J Reprod Immunol 2010; 64: 218-224 Problem We aimed to investigate the expression of the tumor-associated RCAS1 protein in maternal blood of uncomplicated pregnancies. Method of study Maternal blood was obtained from women with uncomplicated pregnancies (N = 43) at 11-13, 20-22, 32-34, 37-38 weeks of gestation, and immediately after delivery. Serum RCAS1 concentration was studied by ELISA, and plasma mRNA was subjected to real-time (RT)-PCR. Results Serum RCAS1 protein concentration was significantly up-regulated at 11-13 and 20-22 weeks than that at 32-34 weeks and after delivery. RCAS1 mRNA level was significantly increased at 11-13 weeks than that at 37-38 weeks. A significant positive correlation was defined between RCAS1 serum concentration at 11-13 weeks and gestational age at delivery and that between plasma RCAS1 mRNA levels at 37-38 weeks and umbilical cord blood base excess. A significant negative correlation was found between RCAS1 serum concentration at 37-38 weeks and umbilical cord blood pH at delivery. Conclusions RCAS1 protein might have importance in the development of uncomplicated pregnancies and for the prediction of pregnancy outcome.
Disciplines :
Reproductive medicine (gynecology, andrology, obstetrics)
Author, co-author :
Tskitishvili, Ekaterine ; Université de Liège - ULiège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement > Osaka University Graduate School of Medicine, Department of Obstetrics and Gynecology
Sharentuya, Namuxila; Osaka University Graduate School of Medicine > Department of Obstetrics and Gynecology
Tsubouchi, Hiroaki; Osaka University Graduate School of Medicine > Department of Obstetrics and Gynecology
Kinugasa-Taniguchi, Yukiko; Osaka University Graduate School of Medicine > Department of Obstetrics and Gynecology
Kanagawa, Takeshi; Osaka University Graduate School of Medicine > Department of Obstetrics and Gynecology
Shimoya, Koichiro; Kawasaki Medical School > Department of Obstetrics and Gynecology
Tomimatsu, Takuji; Osaka University Graduate School of Medicine > Department of Obstetrics and Gynecology
Kimura, Tadashi; Osaka University Graduate School of Medicine > Department of Obstetrics and Gynecology
Language :
English
Title :
Maternal Blood Serum and Plasma Human Tumor-Associated Antigen RCAS1 During the Course of Uncomplicated Pregnancies: A Prospective Study.
Sargent IL, Borzychowski AM, Redman C. NK cells and human pregnancy-an inflammatory view. Trends Immunol 2006, 27:399-404.
Yagel S. The developmental role of natural killer cells at the fetal-maternal interface. Am J Obstet Gynecol 2009, 201:344-350.
Nakashima M, Sonoda K, Watanabe T. Inhibition of cell growth and induction of apoptotic cell death by the human tumor-associated antigen RCAS1. Nat Med 1999, 5:938-942.
Matsushima M, Nakashima M, Ohshima K, Abe Y, Nishimura J, Nawata H, Watanabe T, Muta K. Receptor binding cancer antigen expressed on SiSo cells, a novel regulator of apoptosis of erythroid progenitor cells. Blood 2001, 98:313-321.
Wicherek L, Klimek M, Dutsch-Wicherek M, Kolodziejski L, Skotniczny K. The molecular changes during placental detachment. Eur J Obstet Gynecol Reprod Biol 2006, 125:171-175.
Reimer TA, Anagnostopoulos I, Erdmann B, Lehmann I, Stein H, Daniel P, Dorken B, Rehm A. Reevaluation of the 22-1-1 antibody and its putative antigen, EBAG9/RCAS1, as a tumor marker. BMC Cancer 2005, 5:47.
Sonoda K, Nakashima M, Kaku T, Kamura T, Nakano H, Watanabe T. A novel tumor-associated antigen expressed in human uterine and ovarian carcinomas. Cancer 1996, 77:1501-1509.
Ikeda K, Sato M, Tsutsumi O, Tsuchiya F, Tsuneizumi M, Emi M, Imoto I, Inazawa J, Muramatsu M, Inoue S. Promoter analysis and chromosomal mapping of human EBAG9 gene. Biochem Biophys Res Commun 2000, 273:654-660.
Tsuchiya F, Ikeda K, Tsutsumi O, Hiroi H, Momoeda M, Taketani Y, Muratasu M, Inoue S. Molecular cloning and characterization of mouse EBAG9, homolog of a human cancer associated surface antigen: expression and regulation by estrogen. Biochem Biophys Res Commun 2001, 284:2-10.
Akahira JI, Aoki M, Suzuki T, Moriya T, Niikura H, Ito K, Inoue S, Okamura K, Sasano H, Yaegashi N. Expression of EBAG9/RCAS1 is associated with advanced disease in human epithelial ovarian cancer. Br J Cancer 2004, 90:2197-2202.
Chatterjee M, Mohapatra S, Ionan A, Bawa G, Ali-Fehmi R, Wang X, Nowak J, Ye B, Nahhas FA, Lu K, Witkin SS, Fishman D, Munkarah A, Morris R, Levin NK, Shirley NN, Tromp G, Abrams J, Draghici S, Tainsky MA. Diagnostic markers of ovarian cancer by high-throughput antigen cloning and detection on arrays. Cancer Res 2006, 66:1181-1190.
Giaginis C, Giagini A, Theocharis S. Receptor-binding cancer antigen expressed on SiSo cells (RCAS1): a novel biomarker in the diagnosis and prognosis of human neoplasia. Histol Histopahol 2009, 24:761-776.
Kaku T, Sonoda K, Kamura T, Hirakawa T, Sakai K, Amada S, Ogawa S, Kobayashi H, Nakashima M, Watanabe T, Nakano H. The prognostic significance of tumor-associated antigen 22-1-1 expression in adenocarcinoma of the uterine cervix. Clin Cancer Res 1999, 5:1449-1453.
Sonoda k, Miyamato S, Yamazaki A, Kobayashi H, Nakashima M, Mekada E, Wake N. Biologic significance of receptor-binding cancer antigen expressed on SiSo cells (RCAS1) as a pivotal regulator of tumor growth through angiogenesis in human tumor uterine cancer. Cancer 2007, 110:1979-1990.
Han Y, Qin W, Huang G. Knockdown of RCAS1 expression by RNA interference recovers T cell growth and proliferation. Caner Lett 2007, 257:182-190.
Sonoda K, Miyamoto S, Yotsumoto F, Yagi H, Nakashima M, Watanabe K, Nakano H. Clinical significance of RCAS1 as a biomarker of ovarian cancer. Oncol Rep 2007, 17:623-628.
Wicherek L, Dutsch M, Mak P, Klimek M, Skladzien J, Dubin A. Comparative analysis of RCAS1 level in neoplasms and placenta. Acta Biochim Pol 2003, 50:1187-1194.
Ohshima K, Nakashima M, Sonoda K, Kikuchi M, Watanabe T. Expression of RCAS1 and FasL in human trophoblasts and uterine glands during pregnancy: the possible role in immune privilege. Clin Exp Immunol 2001, 123:481-486.
Wicherek L, Dutsch-Wicherek M, Mak P, Klimek M. The Role of RCAS1 and oxytocinase in immune tolerance during pregnancy. Fetal Diagn Ther 2005, 20:420-425.
Wicherek L, Klimek M, Dutsch-Wicherek M. The level of maternal immune tolerance and fetal maturity. Neuro Endocrinol Lett 2005, 26:561-566.
Galazka K, Wicherek L, Sikora J, Czekierdowski A, Banas T, Bednarek W, Obrzut B, Blecharz P, Reron A, Kalinka J. RCAs1 decidual immunoreactivity during stillbirth: immune cell presence and activity. Am J Reprod Immunol 2008, 60:513-522.
Wicherek L, Klimek M, Czekierdowski A, Popiela TJ, Galazka K, Tetlak T, Gilowski A, Dutsch-Wicherek M. The placental RCAS1 expression during stillbirth. Reprod Biol Endocrinol 2005, 3:24.
Tskitishvili E, Komoto Y, Kinugasa Y, Kanagawa T, Song M, Mimura K, Tomimatsu T, Kimura T, Shimoya K. Relationship between human tumor-associated antigen RCAS1 and gestational diabetes mellitus. Am J Reprod Immunol 2007, 58:440-446.
Tskitishvili E, Komoto Y, Kinugasa Y, Kanagawa T, Song M, Mimura K, Tomimatsu T, Kimura T, Shimoya K. The human tumor-associated antigen RCAS1 in pregnancies complicated by preeclampsia. J Reprod Immunol 2008, 77:100-108.
Tskitishvili E, Nakamura H, Kinugasa-Taniguchi Y, Kanagawa T, Kimura T, Tomimatsu T, Shimoya K. Temporal and spatial expression of tumor-associated antigen RCAS1 in pregnant muse uterus. Am J Reprod Immunol 2010, 63:137-143.
Ng EK, Tsui NB, Lau TK, Leung TN, Chiu RW, Panesar NS, Lit LC, Chan KW, Lo YM. mRNA of placental origin is readily detectable in maternal plasma. Proc Natl Acad Sci USA 2003, 100:4748-4753.
Lo DYM, Chiu RWK. The biology and diagnostic applications of plasma RNA. Ann NY Acad Sci 2004, 1022:135-139.