Reference : Determination of Alpha-Solanine and Alpha-Chaconine in Potatoes by High-Performance T...
Scientific journals : Article
Human health sciences : Pharmacy, pharmacology & toxicology
Determination of Alpha-Solanine and Alpha-Chaconine in Potatoes by High-Performance Thin-Layer Chromatography/Densitometry
Bodart, P. [> > > >]
Kabengera, C. [> > > >]
Noirfalise, Alfred mailto [Université de Liège - ULiège > Services généraux (Faculté de médecine) > Relations académiques et scientifiques (Médecine) >]
Hubert, Philippe mailto [Université de Liège - ULiège > > Chimie analytique >]
Angenot, Luc mailto [Université de Liège - ULiège > Département de pharmacie > Pharmacognosie >]
Journal of AOAC International
6, Nov-Dec
Yes (verified by ORBi)
[en] A high-performance thin-layer chromatographic (HPTLC) method was used to determine the glycoalkaloids alpha-solanine and alpha-chaconine in potatoes. Alpha-solanine and alpha-chaconine are extracted from dehydrated potatoes with boiling methanol-acetic acid (95 + 5, v/v). The analytes are separated on a Silica Gel 60 F254 HPTLC plate by a saturated mixture of dichloromethane-methanol-water-concentrated ammonium hydroxide (70 + 30 + 4 + 0.4, v/v), which is used for vertical development of the plate up to a distance of 85 mm. For visualization, the plate is dipped 3 times into a modified Carr-Price reagent, 20% (w/v) antimony(III) chloride in acetic acid-dichloromethane (1 + 3, v/v), and subsequently heated on a hot plate at 105 degrees C for 5 min. The glycoalkaloids all appear as red chromatographic zones on a colorless background. Densitometric quantification is performed at 507 nm by reflectance scanning. After determination of the appropriate response function, the proposed method was validated. Good results with respect to linearity, accuracy, and precision were obtained in the concentration range studied.
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