Clostridium perfringens urease genes are plasmid-borne.

[en] Although many bacteria are ureolytic, and in some cases urease acts as a virulence factor, the urease phenotype has not been analyzed in the anaerobic pathogen Clostridium perfringens. In this study, ;2% of C. perfringens strains, representing the principal biotypes, were found to harbor the urease structural genes, ureABC, and these were localized on large plasmids that often encode, in addition, the lethal « or i toxins or the enterotoxin. This represents the first report of a plasmid-encoded urease in a gram-positive bacterium. The C. perfringens enzyme was highly similar to the ureases of other bacteria and cross-reacted with antibodies raised against the urease purified from Helicobacter pylori. Urease production was inhibited by urea and induced under growth conditions where the availability of nitrogen sources was limiting. To date, this form of regulation has been observed only for chromosomal ureABC genes.

Disciplines :

Food science

Author, co-author :

Dupuy, B.

Daube, Georges ; Université de Liège - ULiège > Département de sciences des denrées alimentaires > Microbiologie des denrées alimentaires

Popoff, M. R.

Cole, S. T.

Language :

English

Title :

Clostridium perfringens urease genes are plasmid-borne.

Publication date :

1997

Journal title :

Infection and Immunity

ISSN :

0019-9567

eISSN :

1098-5522

Publisher :

American Society for Microbiology (ASM), Washington, United States - District of Columbia

Volume :

Pages :

2313-2320

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 28 February 2009

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