The enterotoxin gene (cpe) of Clostridium perfringens can be chromosomal or plasmid-borne

[en] The location of the cpe gene, encoding the enterotoxin responsible for food poisoning in humans, has been studied in a series of enterotoxigenic Ciostridium perfringens strains by means of pulsed field gel electrophoresis of genomic DNA. The cpe gene was found at the same chromosomal locus in strains associated with food poisoning in humans and was shown to be linked to a repetitive sequence, the Hin dlll repeat, and an open reading frame, ORF3, that may be part of an insertion sequence. In contrast, when the strains originated from domesticated livestock cpe was located on a large episome where it was often close to a copy of the transposable element IS 1151. In these cases, the Hin dlll repeat was not linked to the cpe gene although this was generally preceded by ORF3

Disciplines :

Microbiology

Author, co-author :

Cornillot, Emmanuel

Saint-Joanis, Brigitte

Daube, Georges ; Université de Liège - ULiège > Département de sciences des denrées alimentaires > Microbiologie des denrées alimentaires

Canard, Bruno

Granum, Per Einar

Cole, Stewart T.

Language :

English

Title :

The enterotoxin gene (cpe) of Clostridium perfringens can be chromosomal or plasmid-borne

Publication date :

1995

Journal title :

Molecular Microbiology

ISSN :

0950-382X

eISSN :

1365-2958

Publisher :

Blackwell Publishing, Oxford, United Kingdom

Volume :

Pages :

639-647

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 28 February 2009

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Bibliography

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