Syngeneic Grafting of Adult Rat Drg-Derived Schwann Cells to the Injured Spinal Cord

[en] A subdural inflatable micro-balloon was used to induce closed traumatic contusion to adult rat spinal cord. This spinal cord injury model was associated with reproducible and graded neurological deficits and histopathological alterations. At various delays after injury, transplantations of syngeneic adult cultured dorsal root ganglion-derived Schwann cells were performed into the spinal cord lesion. The transplants were well integrated and reduced the microcystic posttraumatic cavitation as well as the gliosis. Schwann cells transplants were invaded by numerous regenerating neurites most of which, based upon their neurotransmitter contents, seem to originate from the dorsal root ganglion.

Disciplines :

Surgery

Author, co-author :

Martin, Didier ; Université de Liège - ULiège > Département des sciences cliniques > Neurochirurgie

Schoenen, Jean ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Neuro-anatomie

Delree, P.

Rigo, Jean-Michel

Rogister, Bernard ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie générales, et biochimie humaine

Leprince, Pierre ; Université de Liège - ULiège > CNCM/ Centre fac. de rech. en neurobiologie cell. et moléc.

Moonen, Gustave ; Université de Liège - ULiège > Neurologie Sart Tilman

Language :

English

Title :

Syngeneic Grafting of Adult Rat Drg-Derived Schwann Cells to the Injured Spinal Cord

Publication date :

1993

Journal title :

Brain Research Bulletin

ISSN :

0361-9230

eISSN :

1873-2747

Publisher :

Elsevier, Netherlands

Volume :

Issue :

3-4

Pages :

507-14

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 27 February 2009

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Bibliography

Bandtlow, Zachleder, Schwab (1990) Oligodendrocytes arrest neurite growth by contact inhibition. J. Neurosci. 10:3837-3848.
Benzel, Lancon, Bairnsfather, Kesterson (1990) Effect of dosage and timing of administration of naloxone on outcome in the rat ventral compression model of spinal cord injury. Neurosurgery 27:597-601.
Blight (1991) Morphometric analysis of a model of spinal cord injury in guinea pigs, with behavioral evidence of delayed secondary pathology. J. Neurol. Sci. 103:156-171.
Bracken, Shepard, Collins, Holford, Young, Baskin, Eisenberg, Flamm, Leo-Summers, Maroon, Marshall, Perot, Piepmeier, Sonntag, Wagner, Wilberger, Winn (1990) A randomized, controlled trial of methylprednisolone or naloxone in the treatment of acute spinal-cord injury. N. Engl. J. Med. 322:1405-1411.
Braughler, Hall, Means, Waters, Anderson (1987) Evaluation of an intensive methylprednisolone sodium succinate dosing regimen in experimental spinal cord injury. J. Neurosurg. 67:102-105.
Bresnahan, Beattie, Stokes, Conway (1991) Three-dimensional computer-assisted analysis of graded contusion lesions in the spinal cord of the rat. J. Neurotrauma 8:91-101.
Caroni, Schwab (1988) Antibody against myelin-associated inhibitor of neurite growth neutralize nonpermissive substrate properties of CNS white matter. Neuron 1:85-96.
Chang, Capone, Brown (1990) Autologous fibroblast implantation: Feasability and potential problems in gene replacement therapy. Mol. Biol. Med 7:461-470.
Choi (1988) Calcium-mediated neurotoxicity: Relationship to specific channel types and role in ischemie damage. Trends Neurosci. 11(10):465-469.
Delrée, Leprince, Schoenen, Moonen (1989) Purification and culture of adult rat dorsal root ganglia neurons. J. Neurosci. Res. 23(2):198-206.
Fowl, Patterson, Gewirtz, Anderson (1990) Protection against postischemic spinal cord injury using a new 21-aminosteroid. J. Surg. Res. 48:597-600.
Grabitz, Freye, Prior, Kolvenbach, Sandmann (1990) The role of Superoxide dismutase (SOD) in preventing postischemic spinal cord injury. Adv. Exp. Med. Biol. 264:13-16.
Hall (1988) Effects of the 21-aminosteroid U-740006F on posttraumatic spinal cord ischemia in cats. J. Neurosurg. 68:462-465.
Hall, Yonkers, McCall, Braughler (1988) Effects of the 21-aminosteroid U74006F on experimental head injury in mice. J. Neurosurg. 68:456-461.
Hefti (1986) Nerve growth factor promotes survival of septal cholinergic neurons after fimbrial transection. J. Neurosci. 6:2155-2162.
Houle, Reier (1988) Transplantation of fetal spinal cord tissue into the chronically injured adult rat spinal cord. J. Comp. Neurol. 269:535-547.
Ikeda, Long (1990) The molecular basis of brain injury and brain edema: The role of oxygen free radical. Neurosurgery 27:1-11.
Joosten, van der Yen, Hooiveld, ten Donkelaar (1991) Induction of corticospinal target finding by release of a diffusible, chemotropic factor in cervical spinal grey matter. Neurosci. Lett. 128:25-28.
Kim, Goldberg, Choi (1987) High concentrations of naloxone attenuate N-methyl-D-aspartate receptor-mediated neurotoxicity. Eur. J. Pharmacol. 138:133-136.
Martin, Delrée, Schoenen, Rogister, Rigo, Leprince, Stevenaert, Moonen (1991) Transplants of syngeneic adult dorsal root ganglion neurons to the spinal cord of rats with acute traumatic paraplegia: Morphological analyses. Restor. Neurol. Neurosci. 2:303-308.
Martin, Schoenen, Delré, Gilson, Rogister, Leprince, Stevenaert, Moonen (1992) Experimental acute traumatic injury of the adult rat spinal cord by a subdural inflatable balloon. J. Neurosci. Res. 32:539-550.
Martin, Schoenen, Delrée, Leprince, Rogister, Moonen (1991) Grafts of syngenic cultured, adult DRG-derived Schwann cells to the injured spinal cord of adult rats: Preliminary morphological studies. Neurosci. Lett. 124:44-48.
Mesulam (1978) Tetramethyl benzidine for horseradish peroxidase neurochemistry: A non-carcinogenic blue reaction-product with superior sensitivity for visualizing neural afferents and efferents. J. Histochem. Cytochem. 26:106-117.
Price, Turner, Cepko (1987) Lineage analysis in the vertebrate nervous system by retrovirus-mediated gene transfer. Proc. Natl. Acad. Sci. USA 84:156-160.
Privat, Mansour, Pavy, Geffard, Sandillon (1986) Transplantation of dissociated foetal serotonin neurons into the transected spinal cord of adult rats. Neurosci. Lett. 66:61-66.
Privat, Mansour, Rajaofetra, Geffard (1989) Intraspinal transplants of serotonergic neurons in the adult rat. Brain Res. Bull. 22:123-129.
Rivlin, Tator (1977) Objective clinical assessment of motor function after experimental spinal cord injury in the rat. J. Neurosurg. 47:577-581.
Savio, Schwab (1990) Lesioned corticospinal tract axons regeneration in myelin-free rat spinal cord. Proc. Natl. Acad. Sci. USA 87:4130-4133.
Schoenen, Delrée, Leprince, Moonen (1989) Neurotransmitter phenotype plasticity in cultured dissociated adult rat dorsal root ganglia: An immunocytochemical study. J. Neurosci. Res. 22(4):473-487.
Schwab (1990) Myelin-associated inhibitors of neurite growth. Exp. Neurol. 109:2-5.
Siesjö, Bengtsson, Grampp, Theander (1989) Calcium excitotoxins and neuronal death in the brain. Annals of the New York Academy of Sciences 568:234-251.
Southern, Berg (1982) Mammalian cell transformation with SV40 hybrid plasmid vectors. Eukaryotic viral vectors , Y. Gluzman, Cold Spring Harbor, New York; 41-45.
Tarlov Spinal cord compression mechanisms of paralysis and treatment, Charles C. Thomas, Springfield, IL; 1957.
Tecoma, Monyer, Goldberg, Choi (1989) Traumatic neuronal injury in vitro is attenuated by NMDA antagonists. Neuron 2:1541-1545.
Weiss, Hartley, Koh, Choi (1990) The calcium channel blocker nifedipine attenuates slow excitatory amino acid neurotoxicity. Science 247:1474-1477.
Yakovleff, Roby-Brami, Guezard, Mansour, Bussel, Privat (1989) Locomotion in rats transplanted with noradrenergic neurons. Brain Res. Bull. 22:115-121.