[en] In this study, robust antibacterial coatings were created on stainless steel through the covalent grafting of antibacterial peptides onto an organic-polymeric interlayer deposited by RF-glow discharge plasma. X-Ray photoelectron spectroscopy was used to characterize and optimize the two steps of the coating process. The biocidal activity of these surfaces was demonstrated against both Gram+ and Gram- bacteria using ISO tests. 3 to 6 log10 reductions of both Gram+ and Gram- bacterial strains were obtained compared to uncoated stainless steel and depending on the particular antibacterial peptide immobilized. Importantly the antibacterial surfaces were resistant to several cleaning conditions. The latter is significant as the stability of such antibacterial surfaces in close to real life conditions is a major concern and leaching, de-lamination, rearrangement and ageing of the coating can lead to insufficient long term biofilm resistance of the surface.
Research center :
Giga-Systems Biology and Chemical Biology - ULiège Ian Wark Research Institute, University of South Australia ArcelorMittal Research Liège
Vreuls, Christelle ; Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Zocchi, Germaine ; Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Thierry, Benjamin; Ian Wark Research Institute, University of South Australia
Garitte, Geoffrey ; Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Griesser, Stefani S; Ian Wark Research Institute, University of South Australia
Archambeau, Catherine; ArcelorMittal Research Liège > New coating technologies
Van de Weerdt, Cécile ; Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Martial, Joseph ; Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Griesser, Hans; Ian Wark Research Institute, University of South Australia
Language :
English
Title :
Prevention of bacterial biofilms by covalent immobilization of peptides onto plasma polymer functionalized substrates
Publication date :
2010
Journal title :
Journal of Materials Chemistry
ISSN :
0959-9428
eISSN :
1364-5501
Publisher :
Royal Society of Chemistry, Cambridge, United Kingdom
Volume :
20
Pages :
8092 - 8098
Peer reviewed :
Peer Reviewed verified by ORBi
Name of the research project :
Biocoat
Funders :
DGTRE - Région wallonne. Direction générale des Technologies, de la Recherche et de l'Énergie [BE] F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
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