Reference : Post-ischemic hypothermia reduced IL-18 expression and suppressed microglial activati...
Scientific journals : Article
Human health sciences : Reproductive medicine (gynecology, andrology, obstetrics)
http://hdl.handle.net/2268/69996
Post-ischemic hypothermia reduced IL-18 expression and suppressed microglial activation in the immature brain.
English
Fukui, On [Osaka University Graduate School of Medicine > Department of Obstetrics and Gynecology > > >]
Kinugasa, Yukiko [Osaka University Graduate School of Medicine > Department of Obstetrics and Gynecology > > >]
Fukuda, Aya [Osaka University Graduate School of Medicine > Department of Obstetrics and Gynecology > > >]
Fukuda, Hirotsugu [Osaka University Graduate School of Medicine > Department of Obstetrics and Gynecology > > >]
Tskitishvili, Ekaterine mailto [Université de Liège - ULiège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement > > Osaka University Graduate School of Medicine, Department of Obstetrics and Gynecology >]
Hayashi, Shusaku [Osaka University Graduate School of Medicine > Department of Obstetrics and Gynecology > > >]
Song, Mihyon [Kawasaki Medical School > Department of Obstetrics and Gynecology > > >]
Kanagawa, Takeshi [Osaka University Graduate School of Medicine > Department of Obstetrics and Gynecology > > >]
Hosono, Takayoshi [Osaka University Graduate School of Medicine > Department of Obstetrics and Gynecology > > >]
Shimoya, Koichiro [Kawasaki Medical School > Department of Obstetrics and Gynecology > > >]
Murata, Yuji [Osaka University Graduate School of Medicine > Department of Obstetrics and Gynecology > > >]
2006
Brain Research
Elsevier Science
1121
1
35-45
Yes (verified by ORBi)
International
0006-8993
Amsterdam
The Netherlands
[en] Animals ; Brain/growth & development ; Brain Ischemia/pathology/physiopathology ; DNA Primers ; Disease Models, Animal ; Female ; Gene Expression Regulation ; Hypothermia/etiology/pathology/physiopathology ; Hypoxia, Brain/pathology/physiopathology ; Interleukin-18/genetics ; Male ; Microglia/pathology/physiology ; Rats ; Rats, Sprague-Dawley ; Reverse Transcriptase Polymerase Chain Reaction ; Temperature
[en] Inflammation is an important factor for hypoxia-ischemia (HI) brain injury. Interleukin (IL)-18 is a proinflammatory cytokine which may be a contributor to injury in the immature brain after HI. To investigate the effects of post-HI hypothermia on IL-18 in the developing brain, 7-day-old rats were subjected to left carotid artery ligation followed by 8% oxygen for 60 min and divided into a hypothermia group (rectal temperature 32 degrees C for 24 h) and a normothermia group (36 degrees C for 24 h). The IL-18 mRNA was analyzed with real-time RT-PCR, and the protein level was analyzed by Western blot, and the location and source of IL-18 were assessed by immunohistochemistry. The significant increase of the IL-18 mRNA was observed in the ipsilateral hemispheres of the normothermia group at 24 h and 72 h after HI compared with controls, but the level in the ipsilateral hemispheres of the hypothermia group was significantly reduced at both time points, compared with the normothermia group, respectively. The IL-18 protein level in the ipsilateral hemispheres of the normothermia group significantly increased at 72 h after HI compared with controls, however, the protein level of the hypothermia group was significantly decreased, compared with the normothermia group. IL-18-positive cells were observed throughout the entire cortex, corpus callosum (CC) and striatum in the ipsilateral hemispheres of normothermia group at 72 h after HI, however, little positive cells were observed in the hypothermia group. Double labeling immunostaining found that most of the IL-18-positive cells were colocalized with lectin, which is a marker of microglia. The number of ameboid microglia (AM) in the normothermia group was significantly increased in cortex and CC, compared with the number in controls, but there were very few ramified microglia (RM) in these areas. In contrast, the number of AM in the hypothermia group was significantly decreased in cortex and CC, compared with the number in the normothermia group, and there were no significant differences in the number of AM and RM between the hypothermia group and controls. In conclusion, we found that IL-18 mRNA and the protein level were attenuated by post-HI hypothermia and that post-HI hypothermia may decrease microglia activation in the developing brain.
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/69996
10.1016/j.brainres.2006.08.121

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Post-ischemic hypothermia reduced IL-18 expression and suppressed microglial activation in the immature brain.pdfThis is an electronic version (Author’s preprint) of an article published in Brain Research ;2006 Nov 22;1121(1):35-45.The original published version is available at: http://www.sciencedirect.com/science/journal/00068993 Author preprint507.94 kBRequest copy

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