Reference : Tritrophic interactions among Macrosiphum euphorbiae aphids, their host plants and en...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Life sciences : Entomology & pest control
Tritrophic interactions among Macrosiphum euphorbiae aphids, their host plants and endosymbionts: investigation by a proteomic approach.
Francis, Frédéric mailto [Université de Liège - ULiège > Sciences agronomiques > Entomologie fonctionnelle et évolutive >]
Guillonneau, F. [> > > >]
Leprince, Pierre mailto [Université de Liège - ULiège > > GIGA - Neurosciences >]
De Pauw, Edwin mailto [Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.) >]
Haubruge, Eric mailto [Université de Liège - ULiège > Services administratifs généraux > Vice-Recteur de Gembloux Agro Bio Tech - Entomologie fonctionnelle et évolutive >]
Jia, L. [> > > >]
Goggin, F. L. [> > > >]
Journal of Insect Physiology
Yes (verified by ORBi)
United Kingdom
[en] Animals ; Aphids/chemistry/microbiology/physiology ; Bacterial Proteins/isolation & purification ; Cytoskeletal Proteins/analysis/isolation & purification ; Electrophoresis, Gel, Two-Dimensional ; Insect Proteins/isolation & purification ; Lycopersicon esculentum/parasitology ; Molecular Sequence Data ; Plant Proteins/isolation & purification ; Plants, Genetically Modified/chemistry/parasitology ; Polymerase Chain Reaction ; Proteomics ; Rickettsia/chemistry/physiology ; Symbiosis/physiology
[en] The Mi-1.2 gene in tomato confers resistance against certain clones of the potato aphid (Macrosiphum euphorbiae). This study used 2D-DIGE coupled with protein identification by MALDI-TOF-MS to compare the proteome patterns of avirulent and semivirulent potato aphids and their bacterial endosymbionts on resistant (Mi-1.2+) and susceptible (Mi-1.2-) tomato lines. Avirulent aphids had low survival on resistant plants, whereas the semivirulent clone could colonize these plants. Eighty-two protein spots showed significant quantitative differences among the four treatment groups, and of these, 48 could be assigned putative identities. Numerous structural proteins and enzymes associated with primary metabolism were more abundant in the semivirulent than in the avirulent aphid clone. Several proteins were also up-regulated in semivirulent aphids when they were transferred from susceptible to resistant plants. Nearly 25% of the differentially regulated proteins originated from aphid endosymbionts and not the aphid itself. Six were assigned to the primary endosymbiont Buchnera aphidicola, and 5 appeared to be derived from a Rickettsia-like secondary symbiont. These results indicate that symbiont expression patterns differ between aphid clones with differing levels of virulence, and are influenced by the aphids' host plant. Potentially, symbionts may contribute to differential adaptation of aphids to host plant resistance.
Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS ; Fonds de la Recherche Fondamentale Collective d'Initiative des Chercheurs - FRFC
Researchers ; Professionals ; Students
Copyright 2009 Elsevier Ltd. All rights reserved.

File(s) associated to this reference

Fulltext file(s):

Restricted access
Francis2010.pdfPublisher postprint563.35 kBRequest copy

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.