[en] A simple, mechanical method is described for the isolation of preantral follicles bovine foetuses of 220-280 days of gestation. On average, 2918+621 (s.d.) preantral follicles were isolated per ovary. The isolated preantral follicles were characterized on the basis of the morphological appearance of the surrounding granulosa cells, the number of granulosa cell layers, and their diameter. The results show that primordial, primary, and secondary follicles differ morphologically and that they can be classified by their diameter.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Hulshof, S. C. J.; University of Utrecht - Utrecht - The Netherlands > Functional Morphology
Figueiredo, José Ricardo; Université de Liège - ULiège > Faculté de Médecine vétérinaire > IRSIA Research unit
Beckers, Jean-François ; Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction
Bevers, M. M.; University of Utrecht - Utrecht - The Netherlands > Hear Health and Reproduction
Van den Hurk, R.; University of Utrecht - Utrecht - The Netherlands > Fuctional Morphology
Language :
English
Title :
Isolation and characterization of preantral follicles from fetal bovine ovaries
