Murine Matrix Metalloproteinase 9 Gene. 5'-Upstream Region Contains Cis-Acting Elements for Expression in Osteoclasts and Migrating Keratinocytes in Transgenic Mice
[en] Knowledge about the regulation of cell lineage-specific expression of extracellular matrix metalloproteinases is limited. In the present work, the murine matrix metalloproteinase 9 (MMP-9) gene was shown to contain 13 exons, and the 2.8-kilobase pair upstream region was found to contain several common promoter elements including a TATA box-like motif, three GC boxes, four AP-1-like binding sites, an AP-2 site, and three PEA3 consensus sequences that may be important for basic activity of the gene. In order to identify cell-specific regulatory elements, constructs containing varying lengths of the upstream region in front of a LacZ reporter gene were made and studied for expression in transgenic mice generated by microinjection into fertilized oocytes. Analyses of the mice revealed that the presence of sequences between -2722 and -7745 allowed for expression in osteoclasts and migrating keratinocytes, i. e. cells that have been shown to normally express the enzyme in vivo. The results represent the first in vivo demonstration of the location of cell-specific control elements in a matrix metalloproteinase gene and show that element(s) regulating most cell-specific activities of 92-kDa type collagenase are located in the -2722 to -7745 base pair region.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Munaut, Carine ; Université de Liège - ULiège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement
Salonurmi, T.
Kontusaari, S.
Reponen, P.
Morita, T.
Foidart, Jean-Michel ; Université de Liège - ULiège > Département des sciences cliniques > Gynécologie - Obstétrique
Tryggvason, K.
Language :
English
Title :
Murine Matrix Metalloproteinase 9 Gene. 5'-Upstream Region Contains Cis-Acting Elements for Expression in Osteoclasts and Migrating Keratinocytes in Transgenic Mice
Publication date :
10 February 1999
Journal title :
Journal of Biological Chemistry
ISSN :
0021-9258
eISSN :
1083-351X
Publisher :
American Society for Biochemistry and Molecular Biology, Baltimore, United States - Maryland
Birkedal-Hansen, H., Moore, W. G. I., Bodden, M. K., Windsor, L. J., Birkedal-Hansen, B., DeCarlo, A., and Engler, J. A. (1993) Crit. Rev. Oral Biol. Med. 4, 197-250
Charmichael, D. F., Sommer, A., Thompson, R. C., Anderson, D. C., Smith, C. G., Welgus, H. G., and Stricklin, G. P. (1986) Proc. Natl Acad. Sci. U. S. A. 83, 2407-2411
Stetler-Stevenson, W. G., Krutzsch, H. C., and Liotta, L. A. (1989) J. Biol. Chem. 264, 17374-17378
Goldberg, G. I., Warmer, B. L., Grant, G. A., Eisen, A. Z., Wilhelm, S., and He, C. (1989) Proc. Natl. Acad. Sci. U. S. A. 86, 8207-8211
Staskus, P. W., Masiarz, F. R. Pallanck, L. J., and Hawkes, S. P. (1991) J. Biol. Chem. 266, 449-454
Hibbs, M. S., Hasty, K. A., Seyer, J. M., Kang, A. H., and Mainardi, C. L. (1985) J. Biol. Chem. 260, 2493-2500
Goldberg, G. I., Wilhelm, S. M., Kronberger, A., Bauer, E. A., Grant, G. A., and Eisen, A. Z. (1986) J. Biol. Chem. 261, 6600-6605
Hasty, K. A., Pourmotabbed, T. F., Goldberg, G., Thompson, J. P., Spinella, D. G., Stevens, R. M., and Mainardi, C. I. (1990) J. Biol. Chem. 265, 11421-11424
Breathnach, R., Matrisian, L. M., Gesnel, M.-C., Staub, A., and Leroy, P. (1987) Nucleic Acids Res. 15, 1139-1151
Whitham, S. E., Murphy, G., Angel, P., Rahmsdorf, H. J., Smith, B. J., Lyons, A., Harris, T., Reynolds, J. J., Herrlich, P., and Docherty, A. J. P. (1987) Biochem. J. 240, 913-916
Basset, P., Bellocq, J. P., Wolf, C., Stoll, I., Hutin, P., Limocher, J. M., Podhajcer, O. L., Chenard, M. P., Rio, M. C., and Chambon, P. (1990) Nature 348, 699-704
Matrisian, L. M., Glaichenhaus, N., Gesnel, M-C., and Breathnach, R. (1985) EMBO J. 4, 1435-1440
Shapiro, S. D., Griffin, G. L., Gilbert, D. J., Jenkins, N. A., Copeland, N. G., Welgus, H. G., Senior, R. M., and Ley, T. J. (1992) J. Biol. Chem. 267, 4664-4671
Shapiro, S. D., Kobayashi, D. K., and Ley, T. J (1993) J. Biol. Chem. 268, 23824-23829
Salo, T., Liotta, L. A., and Tryggvason, K. (1983) J. Biol. Chem. 258, 3058-3063
Wilhelm, S. M., Collier, I. E., Manner, B. L., Eisen, A. Z., Grant, G. A., and Goldberg, G. I. (1989) J. Biol. Chem. 264, 17213-17221
Collier, I. E., Wilhelm, S. M., Eisen, A. Z., Manner, B. L., Grant, G. A., Seltzer, J. L., Kronberger, A., He, C., Bauer, E. A., and Goldberg, G. I. (1988) J. Biol. Chem. 263, 6579-6587
Huhtala, P., Eddy, R. L., Fan, Y. S., Byers, M. G., Shows, T. B., and Tryggvason, K. (1990) Genomics 6, 554-559
Huhtala, P., Tuuttila, A., Chow, L., Lohi, J., Keski-Oja, J., and Tryggvason, K. (1991) J. Biol. Chem. 266, 16485-16490
Sato, H., Takino, T., Okada, Y., Cao, J., Shinagawa, A., Yamamoto, E., and Seiki, M. (1994) Nature 370, 61-65
Will, H., and Hintzmann, B. (1995) Eur. J. Biochem. 231, 602-608
Takino, T., Sato, H., Shinagawa, A., and Seiki, M. (1995) J. Biol. Chem. 270, 23013-23020
Reponen, P., Sahlberg, C., Huhtala, P., Hurskainen, T., Thesleff, I., and Tryggvason, K. (1992) J. Biol. Chem. 267, 7856-7862
Poulsom, R., Pignatelli, M., Stetler-Stevenson, W. G., Liotta, L. A., Wrigth, P. A., Jeffery, R. E., Longcroft, J. M., Rogers, L., and Stamp, G. W. H. (1992) Am. J. Pathol. 141, 389-396
Pyke, C., Ralfkiaer, E., Huhtala, P., Hurskainen, T., Danø, K., and Tryggvason, K. (1992) Cancer Res. 52, 1336-1341
Pyke, C., Ralfkiaer, E., Danö, K., and Tryggvason, K. (1993) Am. J. Pathol. 142, 359-365
Tryggvason, K., Höyhtyä, M., and Pyke, C. (1993) Breast Cancer Res. Treat. 24, 209-218
Reponen, P., Sahlberg, C., Munaut, C., Thesleff, I., and Tryggvason, K. (1994) J. Cell Biol. 124, 1091-1102
Salo, T., Mäkelä, M., Autio-Harmainen, H., and Larjava, H. (1994) Lab. Invest. 70, 176-182
Mainardi, C. L., Hibbs, M. S., Hasty, K. A., and Seyer, J. M. (1984) Coll. Relat. Res. 4, 479-492
Behrendtsen, O., Alexander, C. M., and Werb, Z. (1992) Development 114, 447-456
Reponen, P., Leivo, I., Sahlberg, C., Apte, S. S., Olsen, B. R., Thesleff, I., and Tryggvason, K. (1995) Dev. Dyn. 202, 388-396
Munaut, C., Reponen, P., Huhtala, P., Kontusaari, S., Foidart, J. M., and Tryggvason, K. (1994) Ann. N. Y. Acad. Sci. 732, 369-371
Sanger, F., Nickten, S., and Coulson, A. R. (1977) Proc. Natl. Acad. Sci. U. S. A. 74, 5463-5467
Chomczynski, P., and Sacchi, N. (1987) Anal. Biochem. 162, 156-159
Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A., and Struhl, A. (1989) John Wiley & Sons, Inc., New York
Hogan, B., Beddington, R., Costantini, F., and Lacy, E. (1986) Manipulating the Mouse Embryo: A Laboratory Manual, pp. 1-497, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
Drews, R., Drohan, W. N., and Lubon, H. (1994) BioTechniques 17, 866-867
Hanley, T., and Merlie, J. P. (1991) BioTechniques 10, 56
Behringer, R. R., Crotty, D. A., Tennyson, V. M., Brinster, R. L., Palmiter, R. D., and Wolgemuth, D. J. (1993) Development 117, 823-833
Bancroft, J. D., and Stevens, A. (1990) Theory and Practice of Histological Techniques (Bancroft, J. D., and Stevens, A., eds) Third Ed., Churchill Livingstone, Edinburgh, UK
Wilkinson, D. G., and Green, J. (1990) in Postimplantation Mammalian Embryos (Copp, A. J., and Cockroft, D. L., eds) pp. 155-171, Oxford University Press, Oxford
Iivanainen, A., Kortesmaa, J., Sahlberg, C., Morita, T., Bergmann, U., Thesleff, I., and Tryggvason, K. (1997) J. Biol. Chem. 272, 27862-27868
Lee, W., Mitchell, P., and Tjian, R. (1987) Cell 49, 741-752
Gutman, A., and Wasylyk, B. (1990) EMBO J. 9, 2241-2246
Masure, S., Nys, G., Fiten, P., Van Damme, J., and Opdenakker, G. (1993) Eur. J. Biochem. 218, 129-141
Fini, E., Bartlett, J., Matsubara, M., Rinehart, W., Mody, M., Girard, M., and Rainville, M. (1994) J. Biol. Chem. 269, 28620-28628
Rossi, P., and Crombrugghe, B. (1987) Proc. Natl. Acad. Sci. U.S.A. 84, 5590-5594
Sato, H., and Seiki, M. (1993) Oncogene 8, 395-405
Sato, H., Kita, M., and Seiki, M. (1993) J. Biol. Chem. 268, 23460-23468
Gum, R., Lengyel, E., Juarez, J., Chen, J. H., Sato, H., Seiki, M., and Boyd, D. (1996) J. Biol. Chem. 271, 10672-10680
Yokoo, T., and Kitamura, M. (1996) J. Physiol. 270, F123-F130
Himelstein, B. P., Lee, E. J., Sato, H., Seiki, M., and Muschel, R. J. (1997) Oncogene 14, 1995-1998
Gum, R., Wang, H., Lengyel, E., Juarez, J., and Boyd, D. (1997) Oncogene 14, 1481-1493
Metsäranta, M., Garofalo, S., Smith, C., Niederreither, K., Crombrugghe, B., and Vuorio. E. (1995) Dev. Dyn. 204, 202-210
Nielsen, B. S., Timshel, S., Kjeldsen, L., Sehested, M., Pyke, C., Borregaard, N., and Danö, K. (1996) Int. J. Cancer 65, 57-62