Article (Scientific journals)
Impaired Accumulation of Granulocytes in the Lung During Ozone Adaptation
Fievez, Laurence; Kirschvink, N.; Dogne, S. et al.
2001In Free Radical Biology and Medicine, 31 (5), p. 633-641
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Keywords :
Ozone; Oxidative stress; H2O2; Inflammation; Neutrophils; Apoptosis; Free radicals
Abstract :
[en] Respiratory alterations induced by an acute exposure to ozone (O(3)) paradoxically resolve during multiday exposure. This adaptation is characteristically accompanied by a gradual attenuation of lung neutrophilia. As maintenance of neutrophilia at the site of inflammation is due to cytokine-mediated delayed neutrophil apoptosis, which is associated with reduced levels of Bax, a proapoptotic protein, we sought to determine whether defects in these mechanisms could account for O(3) adaptation. Lung granulocytes obtained at different time points from calves exposed to 0.75 ppm O(3) for 12 h/d for 7 consecutive days neither showed enhancement of survival nor Bax deficiency, when compared to blood granulocytes. To further investigate the effects of an exogenous oxidative stress on neutrophil survival, human granulocytes were treated with hydrogen peroxide alone, or in combination with granulocyte/macrophage colony-stimulating factor, an antiapoptotic cytokine. Both treatments led to rapid apoptosis associated with downregulation of Bcl-x(L) and Bcl-2, two antiapoptotic proteins. This study shows that O(3) adaptation is associated with a failure in the mechanisms leading to accumulation of neutrophils at the site of inflammation, and suggests that this defect is due to direct proapoptotic effects of exogenous oxidative stress on granulocytes
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Fievez, Laurence ;  Université de Liège - ULiège > Département de sciences fonctionnelles > Biochimie et biologie moléculaire
Kirschvink, N.
Dogne, S.
Jaspar, Fabrice ;  Université de Liège - ULiège > Clinique des grands animaux
Merville, Marie-Paule ;  Université de Liège - ULiège > Département de pharmacie > Chimie médicale
Bours, Vincent ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Génétique générale et humaine
Lekeux, Pierre ;  Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie
Bureau, Fabrice ;  Université de Liège - ULiège > Département de sciences fonctionnelles > Biochimie et biologie moléculaire
Language :
English
Title :
Impaired Accumulation of Granulocytes in the Lung During Ozone Adaptation
Publication date :
01 September 2001
Journal title :
Free Radical Biology and Medicine
ISSN :
0891-5849
eISSN :
1873-4596
Publisher :
Elsevier, Amsterdam, Netherlands
Volume :
31
Issue :
5
Pages :
633-641
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 20 February 2009

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