High-Performance Anion-Exchange Chromatography - Dad As A Tool For The Identification And Quantification Of Oligogalacturonic Acids In Pectin Depolymerisation
Deconinck, Thomas J.-M.; Ciza, Antoine; Sinnaeve, Georgeset al.
2000 • In Carbohydrate Research, 329 (4), p. 907-911
[en] High-performance anion-exchange chromatography (HPAEC) coupled with a diode array detector (DAD) was used to identify and quantify oligogalacturonic acid components in pectins. Purified pectin lyase and polygalacturonase were used to generate unsaturated and saturated oligomers from pectins and sodium polygalacturonate, respectively. This method resulted in a good separation of saturated and unsaturated oligomers up to DP 13. It allowed us to follow polygalacturonase and pectate lyase depolymerisation pathways simultaneously.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Deconinck, Thomas J.-M.; Centre de Recherches Agronomiques de Gembloux > Département Qualité
Ciza, Antoine
Sinnaeve, Georges
Laloux, Jean T.
Thonart, Philippe ; Université de Liège - ULiège > Gembloux Agro-Bio Tech
Language :
English
Title :
High-Performance Anion-Exchange Chromatography - Dad As A Tool For The Identification And Quantification Of Oligogalacturonic Acids In Pectin Depolymerisation
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Campos D.G. Contribution a l'étude de la production et de la caractérisation de la polygalacturonase de Kluyveromyces marxianus et de son mutant KF28 , Dissertation, Faculté des Sciences Agronomiques de Gembloux, Belgium; 1993, 58-64.
