[en] The in vivo ability of the specific PAF-antagonist WEB 2086, a thienotriazolodiazepine, to inhibit platelet-activating factor (PAF) in cattle was investigated by in vitro determination of platelet aggregation curves. WEB 2086 was infused intravenously into a group of 5 healthy male Friesian calves in a dose of 3 mg/kg over 1 min. The resultant inhibition peaked between 30 min and 1 h after administration of WEB 2086. The inhibition was significantly reduced after 3 h and became non-significant after 6 h, but maximal pre-treatment aggregation had not been restored by 24 h after the injection of WEB 2086. These results confirm previous results obtained in vitro and suggest that WEB 2086 is a potent antagonist of PAF activity in calves. They also suggest that further clinical studies with WEB 2086 in cattle are desirable.
Disciplines :
Veterinary medicine & animal health Anatomy (cytology, histology, embryology...) & physiology
Author, co-author :
Bastos da Silva, Miriam; Université de Liège - ULiège > Faculty of Veterinary Medicine > Laboratory of Functional Investigation
Gustin, Pascal ; Université de Liège - ULiège > Faculty of Veterinary Medicine > Laboratory of Pharmacology and Toxicology
Herion, Francine ; Centre Hospitalier Universitaire de Liège - CHU > Thrombosis Homestasis Unit
Raskinet, Renée ; Centre Hospitalier Universitaire de Liège - CHU > Thrombosis Homestasis Unit
David, Jean-Louis ; Centre Hospitalier Universitaire de Liège - CHU > Thrombosis Homestasis Unit
Gougnard, Thierry; Centre Hospitalier Universitaire de Liège - CHU > Laboratory of Clinical Toxicology
Plomteux, Guy ; Centre Hospitalier Universitaire de Liège - CHU > Laboratory of clinical toxicology
Desmecht, Daniel ; Université de Liège - ULiège > Faculty of Veterinary Medicine > Laboratory of Functional Investigation
Lekeux, Pierre ; Université de Liège - ULiège > Faculty of Veterinary Medicine > Laboratory of Functional Investigation
Language :
English
Title :
The Effect of Intravenous Administration of Web 2086 on Paf-Induced Platelet Aggregation in Healthy Friesian Calves
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