Article (Scientific journals)
In vitro effects of oxygen on physico-chemical properties of horse erythrocyte membrane
Portier, Karine; Guichardant, Michel; Debouzy, Jean-Claude et al.
2007In Environmental Toxicology and Pharmacology, 23 (3), p. 340-346
 

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Keywords :
erythrocyte; oxygen; lipid peroxidation; membrane fluidity; 4 HNE
Abstract :
[en] Whether direct exposure to different concentrations (0%, 13%, 100%) of oxygen may affect horse erythrocyte membrane fluidity (EMF) and fatty acid (FA) composition was studied during 1 (T60) and 2 h (T120) exposure. EMF was investigated at the head group level and hydrophobic core thanks to phosphorus nucleus 31 (P-31) nuclear magnetic resonance (P-31 NMR) and electronic paramagnetic resonance (EPR) using two spin probes: 5-nitroxydestearic acid and 16-doxylstearic acid. Lipid structure of the membranes was studied by gas liquid chromatography. 4-Hydroxy-2E-nonenal was also analyzed as a marker of lipid peroxidation. It increased at T120 13% and 100% oxygen whereas there were no significant changes in membrane dynamic or structure. Correlation was demonstrated between EMF and partial pressure of oxygen in the blood (P-O2). In vitro high rate of oxygenation was efficient to induce lipid peroxidation but did not change membrane dynamics. This may be due to a low free radical production in vitro or to the high red blood cells antioxidant properties. (c) 2007 Elsevier B.V. All rights reserved.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Portier, Karine
Guichardant, Michel
Debouzy, Jean-Claude
Crouzier, David
Geraud, Ingrid
Kirschvink, Nathalie
Lekeux, Pierre ;  Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie
Fellmann, Nicole
Coudert, Jean
Language :
English
Title :
In vitro effects of oxygen on physico-chemical properties of horse erythrocyte membrane
Publication date :
May 2007
Journal title :
Environmental Toxicology and Pharmacology
ISSN :
1382-6689
Publisher :
Elsevier Science Bv, Amsterdam, Netherlands
Volume :
23
Issue :
3
Pages :
340-346
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