Electrospray Mass Spectrometry of Telomeric RNA (TERRA) Reveals the Formation of Stable Multimeric G-Quadruplex Structures

Collie, Gavin W.; Parkinson, Gary N.; Neidle, Stephen; Rosu, Frédéric; De Pauw, Edwin; Gabelica, Valérie

doi:10.1021/ja100345z

Article (Scientific journals)

Electrospray Mass Spectrometry of Telomeric RNA (TERRA) Reveals the Formation of Stable Multimeric G-Quadruplex Structures

Collie, Gavin W.; Parkinson, Gary N.; Neidle, Stephen et al.

2010 • In Journal of the American Chemical Society, 132 (27), p. 9328–9334

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/66137

DOI
10.1021/ja100345z

PubMed
20565109

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Keywords :

mass spectrometry; noncovalent; G-quadruplex; DNA; RNA; spectroscopy

Abstract :

[en] We report on the self-assembled structures formed by 12-mer, 22-mer, and 45-mer telomeric RNA (telRNA/TERRA) sequences compared to their DNA analogues, as studied by electrospray mass spectrometry, circular dichroism, and thermal denaturation. The major difference between telomeric RNA and DNA sequences is the ability of telomeric RNA to form higher-order dimeric assemblies, initiated by cation-mediated stacking of two parallel G-quadruplex subunits. The 5′-5′ stacking had been observed recently by NMR for the r(GGGUUAGGGU) 10-mer (Martadinata, H.; Phan, A. T. J. Am. Chem. Soc. 2009, 131, 2570); the present work shows that stacking also occurs for the 22-mer containing four G-tracts and for the 45-mer containing eight G-tracts, suggesting a general structural feature of telomeric RNA. The importance of kinetic effects in multimer formation, unfolding, and structural rearrangements is also highlighted.

Research center :

Giga-Systems Biology and Chemical Biology - ULiège

Disciplines :

Biochemistry, biophysics & molecular biology
Chemistry

Author, co-author :

Collie, Gavin W.; CRUK Biomolecular Structure Group, The School of Pharmacy, UniVersity of London, WC1N 1AX London, United Kingdom

Parkinson, Gary N.; CRUK Biomolecular Structure Group, The School of Pharmacy, UniVersity of London, WC1N 1AX London, United Kingdom

Neidle, Stephen; CRUK Biomolecular Structure Group, The School of Pharmacy, UniVersity of London, WC1N 1AX London, United Kingdom

Rosu, Frédéric ; Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.)

De Pauw, Edwin ; Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.)

Gabelica, Valérie ; Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.)

Language :

English

Title :

Electrospray Mass Spectrometry of Telomeric RNA (TERRA) Reveals the Formation of Stable Multimeric G-Quadruplex Structures

Publication date :

2010

Journal title :

Journal of the American Chemical Society

ISSN :

0002-7863

eISSN :

1520-5126

Publisher :

American Chemical Society, Washington, United States - District of Columbia

Volume :

132

Issue :

Pages :

9328–9334

Peer reviewed :

Peer Reviewed verified by ORBi

Funders :

F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
EU COST action MP0802

Available on ORBi :

since 10 July 2010

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