Reference : Metallo-beta-lactamases (classification, activity, genetic organization, structure, z...
Scientific journals : Other
Life sciences : Biochemistry, biophysics & molecular biology
Metallo-beta-lactamases (classification, activity, genetic organization, structure, zinc coordination) and their superfamily
Bebrone, Carine mailto [Université de Liège - ULiège > > Centre d'ingénierie des protéines >]
Biochemical Pharmacology
Pergamon-Elsevier Science Ltd
Yes (verified by ORBi)
[en] bacterial resistance ; class B beta-lactamases ; zinc binding sites ; alpha beta beta alpha-fold ; conserved motifs ; metallo-beta-lactamase superfamily
[en] One strategy employed by bacterial strains to resist beta-lactam antibiotics is the expression of metallo-beta-lactamases requiring Zn+2 for activity. In the last few years, many new zinc beta-lactamases have been described and several pathogens are now known to synthesize members of this class. Metallo-beta-lactamases are especially worrisome due to: (1) their broad activity profiles that encompass most beta-lactam antibiotics, including the carbapenems; (2) potential for horizontal transference; and (3) the absence of clinically useful inhibitors. on the basis of the known sequences, three different lineages, identified as subclasses B1, B2, and B3 have been characterized. The three-dimensional structure of at least one metallo-p-lactamase of each subclass has been solved. These very similar 3D structures are characterized by the presence of an alpha beta beta alpha-fold. In addition to metallo-beta-lactamases which cleave the amide bond of the beta-lactam ring, the metallo-beta-lactamase superfamily includes enzymes which hydrolyze thiol-ester, phosphodiester and sulfuric ester bonds as well as oxydoreductases. Most of the 6000 members of this superfamily share five conserved motifs, the most characteristic being the His116-X-His118-X-Asp120-His121 signature. They all exhibit an alpha beta beta alpha-fold, similar to that found in the structure of zinc beta-lactamases. Many members of this superfamily are involved in mRNA maturation and DNA reparation. This fact suggests the hypothesis that metallo-beta-lactamases may be the result of divergent evolution starting from an ancestral protein which did not have a beta-lactamase activity. (c) 2007 Elsevier Inc. All rights reserved.
The author acknowledges Biochemical Pharmacology.

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