[en] The aim of this work was to evaluate the efficiency of different embryo culture methods for in vitro embryo production: development in co-culture with bovine oviductal epithelial cells (BOEC), in BOEC conditioned medium (CM) or in rabbit oviducts, versus in vivo produced embryos. There was no significant difference in terms of percentages of cleaved and 8-cell stages obtained between CM and BOEC. In CM, 24.8% of the cleaved embryos became blastocysts. In BOEC, 14.5% of the cleaved embryos became blastocysts. Among the 190 zygotes transferred in the rabbit oviducts, 127 have been recovered 5 days later, and 17.4% became blastocysts. There was no significant difference in term of blastocyst formation between the development in rabbit and in BOEC. However, there was a significant difference between the CM group and the two other groups. The numbers of cells in blastocysts from different sources were investigated: in vivo blastocysts contained 107 cells assumed to be 100%, in vitro blastocysts developed in rabbit oviduct 100.1 cells (93.3%), BOEC blastocysts 90.8 cells (84.6%) and CM blastocysts 72.3 cells (67.3%). This study confirmed earlier works on the oviduct effect on blastocyst quality in terms of development rate and cell number.
Research Center/Unit :
Laboratoire d'Endocrinologie et de Physiologie de la Reproduction animale
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Ectors, Fabien ; Université de Liège - ULiège > Faculté de Médecine Vétérinaire
Thonon, Fabienne; Université de Liège - ULiège > Faculté de Médecine Vétérinaire
Delval, Alain; Université de Liège - ULiège > Faculté de Médecine Vétérinaire
Fontes, Reginaldo S; Université de Liège - ULiège > Faculté de Médecine Vétérinaire
Touati, Kamal ; Université de Liège - ULiège > Faculté de Médecine Vétérinaire
Beckers, Jean-François ; Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction
Language :
English
Title :
Comparison between culture of bovine embryos in vitro versus development in rabbit oviducts and in vivo
Publication date :
1993
Journal title :
Livestock Production Science
ISSN :
0301-6226
Publisher :
Elsevier Science, Amsterdam, Netherlands
Volume :
36
Pages :
29-34
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
IRSIA - Institut pour l'Encouragement de la Recherche Scientifique dans l'Industrie et l'Agriculture [BE]
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