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Poster (Scientific congresses and symposiums)
SYNOVIOCYTES, NOT CHONDROCYTES, RELEASE FREE RADICALS AFTER CYCLES OF ANOXIA/RE-OXYGENATION
Schneider, Nicole; Mouithys-Mickalad, Ange; Lejeune, Jean-Philippe et al.
2007Hippos
 

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Keywords :
equine; osteoarthritis; articular chondrocytes; synoviocytes; free radicals; electronic spin resonance; anoxia; reoxygenation
Abstract :
[en] Introduction : An oxidant activity has been implicated in the onset of equine osteoarthritis. Most of the studies have been done on articular chondrocytes, but little is known about the role of synoviocytes. Objective : Our aim was to investigate if equine articular chondrocytes, primary synoviocytes or synoviocytes of a continuous cell line are able to produce free radicals after exposure to anoxia and re-oxygenation. Methods : By oxymetry and electron paramagnetic resonance (EPR), we investigated the effects of repeated anoxia/re-oxygenation (A/R) periods on the respiration and production of free radicals by synoviocytes (rabbit HIG-82 cell line and primary equine synoviocytes) and equine articular chondrocytes. Three periods of 20 min anoxia followed by re-oxygenation were applied to 10exp7 cells; O2 consumption was measured before anoxia and after each re-oxygenation. After the last A/R, cellular free radical formation was investigated by EPR spectroscopy with spin trapping technique (n = 3 for each cell line). Results : Both types of synoviocytes showed a high O2 consumption, which was slower after anoxia. By EPR with the spin trap POBN, we proved a free radical formation. Results were similar for equine and rabbit synoviocytes. For chondrocytes, we observed a low O2 consumption, unchanged by anoxia, and no free radical production. Conclusion : These observations suggest an oxidant activity of synoviocytes, potentially important for the onset of osteoarthritis.
Research center :
CORD - Centre de l'Oxygène, Recherche et Développement - ULiège
Disciplines :
Veterinary medicine & animal health
Human health sciences: Multidisciplinary, general & others
Author, co-author :
Schneider, Nicole ;  Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Pôle équin, CORD, Centre Européen du Cheval de Mont le Soie
Mouithys-Mickalad, Ange ;  Université de Liège - ULiège > Centre de l'oxygène : Recherche et développement (C.O.R.D.)
Lejeune, Jean-Philippe ;  Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Dupont, Ginette ;  Université de Liège - ULiège > Service d'Anesthésiologie CHU > CORD
Serteyn, Didier  ;  Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Language :
English
Title :
SYNOVIOCYTES, NOT CHONDROCYTES, RELEASE FREE RADICALS AFTER CYCLES OF ANOXIA/RE-OXYGENATION
Publication date :
2007
Event name :
Hippos
Event organizer :
Neo-Animalia
Event place :
Liège, Belgium
Event date :
13./14. janvier 2007
Audience :
International
Funders :
Région wallonne. Ministère de l'Agriculture, de la Nature, de la Forêt, de la Ruralité, du Tourisme, du Patrimoine [BE]
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since 30 June 2010

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