[en] We investigated the oxygen (O2) uptake of equine articular chondrocytes to assess their reactions to anoxia/re-oxygenation. They were cultured under 5% or 21% gas phase O2 and at glucose concentrations of 0, 1.0 or 4.5 g/L in the culture medium (n = 3). Afterwards, the O2 consumption rate of the chondrocytes was monitored (oxymetry) before and after an anoxia period of 25 min. The glucose consumption and lactate release were measured at the end of the re-oxygenation period. The chondrocytes showed a minimal O2 consumption rate, which was hardly changed by anoxia. Independently from the O2 tension, glucose uptake by the cells was about 30% of the available culture medium glucose, thus higher for cells at 4.5 g/L glucose (n = 3). Lactate release was also independent from O2 tension, but lower for cells at 4.5 g/L glucose (n = 3). Our observations indicated that O2 consumption by equine chondrocytes was very low despite a functional mitochondrial respiratory chain, and nearly insensitive to anoxia/re-oxygenation. But the chondrocytes metabolism was modified by an excess of O2 and glucose.
Research center :
Centre of Oxygen Research & Development (CORD), Institut de Chimie, B6a, Sart Tilman, University of Liège, 4000 Liège, Belgium Department of Clinical Sciences, Large Animal Surgery, Faculty of Veterinary Medicine Centre Europeén du Cheval, Mont-le-Soie, Vielsam, Belgium
Disciplines :
Veterinary medicine & animal health Human health sciences: Multidisciplinary, general & others Biochemistry, biophysics & molecular biology
Author, co-author :
Schneider, Nicole ; Université de Liège - ULiège > CORD, Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Mouithys-Mickalad, Ange ; Université de Liège - ULiège > Centre de l'oxygène : Recherche et développement (C.O.R.D.)
Lejeune, Jean-Philippe ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Duyckaerts, Claire ; Université de Liège - ULiège > Centre de l'oxygène : Recherche et développement (C.O.R.D.)
Sluse, Francis ; Université de Liège - ULiège > Département des sciences de la vie > Bioénergétique et physiologie cellulaire
Deby-Dupont, Ginette; Centre de l'oxygène : Recherche et développement (C.O.R.D.)
Serteyn, Didier ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Language :
English
Title :
Oxygen consumption of equine articular chondrocytes: Influence of applied oxygen tension and glucose concentration during culture.
Publication date :
2007
Journal title :
Cell Biology International
ISSN :
1065-6995
eISSN :
1095-8355
Publisher :
Academic Press, London, United Kingdom
Volume :
31
Pages :
878-886
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
Ministère de l’Agriculture et de la Ruralité de la Région Wallonne
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