[en] A method and a device for making cell membranes permeable using electrical discharges (electroporation), wherein at least one short electrical discharge is applied to a suspension containing cells to be made permeable and molecules to be accommodated therein, in order to generate in said suspension a high-value electric field, whereafter at least one longer electrical discharge is applied thereto to generate a lower-value electric field therein.
Disciplines :
Genetics & genetic processes
Inventor :
Servais, Christian ; Université de Liège - ULiège > Département d'astrophys., géophysique et océanographie (AGO) > Groupe infra-rouge de phys. atmosph. et solaire (GIRPAS)
Louette, Joël
Language :
French
Title (54) :
PROCEDE ET DISPOSITIF DE PERMEABILISATION DE CELLULES VIVANTES
Alternative titles :
[en] METHOD AND DEVICE FOR MAKING LIVING CELLS PERMEABLE