[en] The gene encoding the Bacillus sphaericus gamma-D-glutamyl-L-diamino acid endopeptidase II, a cytoplasmic enzyme involved in cell sporulation [1], contains the information for a 271-amino acid protein devoid of a signal peptide. The endopeptidase lacks sequence relatedness with other proteins of known primary structure except that its C-terminal region has significant similarity with the C-terminal region of the 54-kDa P54 protein of Enterococcus faecium, of unknown function [2].
Research Center/Unit :
CIP - Centre d'Ingénierie des Protéines - ULiège
Disciplines :
Microbiology
Author, co-author :
Hourdou, Marie-Laure; Université de Liège - ULiège > Institut de Chimie > Centre d'ingénieurie des Protéines
Duez, Colette ; Université de Liège - ULiège > Centre d'ingénierie des protéines
Joris, Bernard ; Université de Liège - ULiège > Centre d'ingénierie des protéines
Vacheron, Marie-Jeanne; Université Claude Bernard - Lyon 1 - UCLB > Laboratoire de Biochimie microbienne
Guinand, Micheline; Université Claude Bernard - Lyon 1 - UCLB > Laboratoire de Biochimie microbienne
Michel, Georges; Université Claude Bernard - Lyon 1 - UCLB > Laboratoire de Biochimie microbienne
Ghuysen, Jean-Marie ; Université de Liège - ULiège > Centre d'ingénierie des protéines
Language :
English
Title :
Cloning and nucleotide sequence of the gene encoding the γ-D-glutamyl-L-diamino acid endopeptidase II of Bacillus sphaericus
Vacheron, Guinand, Françon, Michel Eur. J. Biochem. 1979, 100:189-196.
Fürst, Mösch, Solioz (1989) A protein of unusual composition fromEnterococcus faecium. Nucleic Acids Research 17:6724.
Moir, Smith Annu. Rev. Microbiol. 1990, 44:531-553.
Bourgogne, Vacheron, Guinand, Michel (1992) Purification and partial characterization of the gamma-D-glutamyl-L-di-amino acid endopeptidase II from Bacillus sphaericus. Int J Biochem, (in press); .
Valentin, Vacheron, Martinez, Guinand, Michel Biochimie 1983, 65:239-245.
Hopwood, Bibb, Chater, Kieser, Bruton, Kieser, Lydiate, Smith, Ward, Schrempf Genetic manipulation of Streptomyces: a laboratory manual, 11th Edn., The John Innes Foundation, Norwich, U.K; 1985.