Abstract :
[en] Novel analogues of the cell-penetrating peptides penetratin and transportan were
synthesized. The distribution of the biotin-labeled peptides in Bowes melanoma
cell line has been investigated by indirect fluorescence with
fluorescein-streptavidin detection. The time course of uptake of (125)I-labeled
transportan analogues has been characterized in the same cell line. Molecular
modeling was used to analyze the penetration and the orientation of molecules in
a simulated biological membrane. The results, both from molecular modeling and
fluorescence studies, imply that penetratin and transportan do not enter the
cells by related mechanisms and that they do not belong to the same family of
translocating peptides.
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