Fusogenic Alzheimer'S Peptide Fragment A Beta (29-42) In Interaction With Lipid Bilayers: Secondary Structure, Dynamics, And Specific Interaction With Phosphatidyl Ethanolamine Polar Heads As Revealed By Solid-State Nmr
[en] The interaction of the native Alzheimer's peptide C-terminal fragment Abeta
(29-42), and two mutants (G33A and G37A) with neutral lipid bilayers made of POPC
and POPE in a 9:1 molar ratio was investigated by solid-state NMR. This fragment
and the lipid composition were selected because they represent the minimum
requirement for the fusogenic activity of the Alzheimer's peptide. The chemical
shifts of alanine methyl isotropic carbon were determined by MAS NMR, and they
clearly demonstrated that the major form of the peptide equilibrated in membrane
is not in a helical conformation. (2)H NMR, performed with acyl chain deuterated
POPC, demonstrated that there is no perturbation of the acyl chain's dynamics and
of the lipid phase transition temperature. (2)H NMR, performed with alanine
methyl-deuterated peptide demonstrated that the peptide itself has a limited
mobility below and above the lipid phase transition temperature (molecular order
parameter equal to 0.94). MAS (31)P NMR revealed a specific interaction with POPE
polar head as seen by the enhancement of POPE phosphorus nuclei T(2) relaxation.
All these results are in favor of a beta-sheet oligomeric association of the
peptide at the bilayer interface, preferentially recruiting phosphatidyl
ethanolamine polar heads.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Ravault, S.
Soubias, O.
Saurel, O.
Thomas, Annick ; Université de Liège - ULiège > Chimie et bio-industries > Centre de Bio. Fond. - Section de Biologie moléc. et numér.
Brasseur, Robert ; Université de Liège - ULiège > Gembloux Agro-Bio Tech
Milon, A.
Language :
English
Title :
Fusogenic Alzheimer'S Peptide Fragment A Beta (29-42) In Interaction With Lipid Bilayers: Secondary Structure, Dynamics, And Specific Interaction With Phosphatidyl Ethanolamine Polar Heads As Revealed By Solid-State Nmr
Publication date :
2005
Journal title :
Protein Science: A Publication of the Protein Society
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