[en] Herpes simplex (HSV) and varicella-zoster (VZV) skin infections share so many histological similarities that distinguishing between them may prove to be impossible. We developed and characterized a new monoclonal antibody, VL8, IgG kappa isotype, directed to the VZV envelope glycoprotein gpI. Immunohistochemistry with VL8 appeared highly sensitive and specific on formalin-fixed paraffin-embedded biopsies and a clear-cut distinction between HSV and VZV infections was possible. The pattern of VL8 immunolabelling in VZV infections was strikingly different from that found in HSV infections studied with polyclonal antibodies to HSV I and II. Double immunolabelling revealed the VL8 positivity of sebaceous cells, endothelial cells, Mac 387-and CD68-positive monocyte-macrophages, and factor XIIIa-positive perivascular, perineural and interstitial dendrocytes. Intracytoplasmic VL8 labelling of endothelial cells and perivascular dendrocytes was found at the site of leukocytoclastic vasculitis.
Disciplines :
Immunology & infectious disease Dermatology
Author, co-author :
Nikkels, Arjen ; Centre Hospitalier Universitaire de Liège – CHU > Dermatopathologie
Debrus, S.
Sadzot-Delvaux, Catherine ; Université de Liège - ULiège > Département des sciences de la vie > Virologie et immunologie - GIGA-M : Coordination scientifique
Piette, Jacques ; Université de Liège - ULiège > Département des sciences de la vie > Virologie - Immunologie
Delvenne, Philippe ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques
Rentier, Bernard ; Université de Liège - ULiège > Département de Microbiologie > Virologie fondamentale et Immunologie
Pierard, Gérald ; Université de Liège - ULiège > Département des sciences cliniques > Dermatopathologie
Language :
English
Title :
Comparative immunohistochemical study of herpes-simplex and varicella-zoster infections
Publication date :
March 1993
Journal title :
Virchows Archiv. A: Pathological Anatomy and Histopathology
ISSN :
0174-7398
Publisher :
Springer verlag
Volume :
422
Issue :
2
Pages :
121-126
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
The original publication is available at http://www.springerlink.com/content/qvw2206455515714/fulltext.pdf
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