[en] Tumor cell-derived collagenase stimulatory factor (TCSF) stimulates in vitro the biosynthesis of various matrix metalloproteinases involved in tumor invasion, such as interstitial collagenase, gelatinase A, and stromelysin 1. The expression of TCSF mRNAs was studied in vivo, using in situ hybridization and Northern blotting analysis, in seven normal tissues and in 22 squamous cell carcinomas of the lung, and in seven benign proliferations and in 22 ductal carcinomas of the mammary gland. By in situ hybridization, TCSF mRNAs were detected in 40 of 44 carcinomas, in pre-invasive and invasive cancer cells of both lung and breast cancers. TCSF mRNAs and gelatinase A mRNAs were both visualized in the same areas in serial sections in breast cancers, and were expressed by different cells, tumor cells, and fibroblasts. The histological results were confirmed by Northern blot analysis, which showed a higher expression of TCSF mRNAs in cancers than in benign and normal tissues. These observations support the hypothesis that TCSF is an important factor in lung and breast tumor progression.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Polette, M.
Gilles, Christine ; Université de Liège - ULiège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement
Marchand, V.
Lorenzato, M.
Toole, B.
Tournier, J. M.
Zucker, S.
Birembaut, P.
Language :
English
Title :
Tumor collagenase stimulatory factor (TCSF) expression and localization in human lung and breast cancers.
Publication date :
1997
Journal title :
Journal of Histochemistry and Cytochemistry
ISSN :
0022-1554
eISSN :
1551-5044
Publisher :
Histochemical Society, New York, United States - New York
Basset P, Bellocq JP, Wolf C, Stoll I, Limacher JM, Podjahcer OL, Chenard MP, Rio MC, Chambon P (1990) A novel metalloproteinase gene specifically expressed in stromal cells of breast carcinomas. Nature 348:699-704
Bauer EA, Uitto J, Walters RC, Eisen AZ (1979) Enhanced collagenase production by fibroblasts derived from human basal cell carcinoma. J Invest Dermatol 39:4594-4599
Bernhard EJ, Muschel RJ, Hughes EN (1990) Mr 92,000 gelatinase release correlates with the metastatic phenotype in transformed rat embryo cells. Cancer Res 50:3872-3877
Biswas C (1985) Matrix influence on the tumor cell stimulation of fibroblast collagenase production. J Cell Biochem 28:39-45
Biswas C (1984) Collagenase stimulation in cocultures of human fibroblasts and human tumor cells. Cancer Lett 24:201-207
Biswas C, Zhang Y, DeCastro R, Guo H, Nakamura T, Kataoka H, Nabeshima K (1995) The human tumor cell-derived collagenase stimulatory factor (renamed EMMPRIN) is a member of the immunoglobin superfamily. Cancer Res 55:434-439
Bonfil RD, Reddel RR, Ura H, Reich R, Fridman R, Harris CC, Klein-Szanto AJP (1989) Invasive and metastatic potential of a v-Ha-ras-transformed human bronchial epithelial cell line. J Natl Cancer Inst 81:587-594
Clavel C, Polette M, Doco M, Binninger I, Birembaut P (1992) Immunolocalization of matrix metalloproteinases and their tissue inhibitor in human mammary pathology. Bull Cancer (Paris) 79:261-270
Davies B, Miles DW, Happerfiled LC, Naylor MS, Bobrow LG, Rubens RD, Balkwill FR (1993) Activity of type IV collagenases in benign and malignant breast disease. Br J Cancer 67:1126-1131
Ellis SM, Nabeshima K, Biswas C (1989) Monoclonal antibody preparation and purification of a tumor cell collagenase-stimulatory factor. Cancer Res 49:3385-3391
Gilles C, Polette M, Piette J, Birembaut P, Foidart JM (1994) Epithelial-to-mesenchymal transition in HPV-33-transfected cervical keratinocytes is associated with increased invasiveness and expression of gelatinase A. Int J Cancer 59:661-666
Gilles C, Polette M, Piette J, Munaut C, Thompson EW, Birembaut P, Foidart JM (1996) High level of MT-MMP expression is associated with invasiveness of cervical cancer cells. Int J Cancer 65:209-213
Hernandez AD, Hibbs MS, Postlethwaite AE (1985) Establishment of basal cell carcinomas in culture: evidence for a basal cell carcinoma-derived factor(s) which stimulates fibroblasts to proliferate and release collagenase. J Invest Dermatol 85:470-475
Hewitt RE, Leach IH, Powe DG, Clark IM, Cawston TE, Turner DR (1991) Distribution of collagenase and tissue inhibitor of metalloproteinases (TIMP) in colorectal tumors. Int J Cancer 49:666-672
Ito A, Nakajima S, Sasaguri Y, Nagase H, Morri Y (1995) Co-culture of human breast adenocarcinoma MCF7 cells and human dermal fibroblasts enhances the production of matrix metalloproteinases 1, 2, and 3 in fibroblasts. Br J Cancer 71:1039-1045
Karinrerk W, Fieberger E, Steffanova I, Baumruker T, Knapp W, Stockinger H (1992) Human leucocyte activation antigen M6, a member of the Ig superfamily, is the species homologue of rat OX-47, more basigin, and chicken HT7 molecule. J Immunol 149:847-854
Kataoka H, DeCasto R, Zucker S, Biswas C (1993) Tumor cell-derived collagenase-stimulatory factor increases expression of interstitial collagenase, stromelysin, and 72-kDa gelatinase. Cancer Res 53:3154-3158
Levy AT, Cioce V, Sobel ME, Garbisa S, Grigioni WF, Liotta LA, Stetler-Stevenson WG (1991) Increased expression of the Mr 72,000 type IV collagenase in human colonic adenocarcinoma. Cancer Res 51:439-444
Monsky WL, Lin CYL, Aoyama A, Kelly T, Akiyama SK, Mueller SC, Chen WT (1994) A potential marker protease of invasiveness, seprase, is localized on invadopodia of human malignant melanoma cells. Cancer Res 54:5702-5710
Monteagudo C, Merino M, San-Juan J, Liotta L, Stetler-Stevenson WG (1990) Immunohistochemical distribution of type IV collagenase in normal, benign, and malignant breast tissue. Am J Pathol 136:585-592
Muller D, Breathnach R, Engelmann A, Mill R, Bronner G, Flesch H, Dumont P, Eber M, Abecassis J (1991) Expression of collagenase-related metalloproteinase genes in human lung or neck tumors. Int J Cancer 48:550-556
Muraoka K, Nabeshima K, Murayama T, Biswas C, Koono M (1993) Enhanced expression of a tumor-cell-derived collagenase-stimulatory factor in urothelium carcinoma: its usefulness as a tumor marker for bladder cancers. Int J Cancer 55:19-26
Murphy G, Segain JP, O'Shea M, Cockett M, Ionnou C, Lefebvre O, Chambon P, Basset P (1994) The 28-kDa N-terminal domain of mouse stromelysin-3 has the general properties of a weak metalloproteinase. J Biol Chem 268:15435-15441
Nabeshima K, Lane WS, Biswas C (1991) Partial sequencing and characterization of the tumor cell-derived collagenase stimulatory factor. Arch Biochem Biophys 285:90-96
Noël A, Munaut C, Nusgens B, Lapière CM, Foidart JM (1993) Different mechanisms of extracellular matrix remodelling by fibroblasts in response to human mammary neoplastic cells. Invasion Metastasis 13:72-81
Noël A, Polette M, Lewalle JM, Munaut C, Emonard H, Birembaut P, Foidart JM (1994) Coordinate enhancement ot gelatinase A mRNA and activity levels in human fibroblasts in response to breast-adenocarcinoma cells. Int J Cancer 56:331-336
Okada A, Bellocq JP, Rouyer N, Chenard MP, Rio MC, Chambon P, Basset P (1995) Membrane-type matrix metalloproteinase (MT-MMP) gene is expressed in stromal cells of human colon, breast, and head and neck carcinomas. Proc Natl Acad Sci USA 92:2730-2734
Polette M, Clavel C, Cockett M, Girod de Bentzmann S, Murphy G, Birembaut P (1993) Detection and localization of mRNAs encoding matrix metalloproteinases and their tissue inhibitor in human breast pathology. Invasion Metastasis 13:31-37
Polette M, Clavel C, Muller D, Abecassis J, Binninger I, Birembaut P (1991) Detection of mRNAs encoding collagenase I and stromelysin 2 in carcinomas of the head and neck by in situ hybridization. Invasion Metastasis 11:76-83
Polette M, Gilbert N, Stas I, Nawrocki B, Noël A, Remacle A, Stetler-Stevenson WG, Birembaut P, Foidart JM (1994) Gelatinase A expression and localization in human breast cancers. An in situ hybridization study and immunohistochemical detection using confocal microscopy. Virchows Arch 424:641-645
Polette M, Nawrocki B, Gilles C, Sato H, Seiki M, Tournier JM, Birembaut P (1996) MT-MMP expression and localisation in human lung and breast cancers. Virchows Arch 428:29-35
Poulsom K, Hanby AM, Pignatelli M, Jeffrey RE, Longcroft JM, Rogers L (1993) Expression of gelatinase A and TIMP2 mRNAs in desmoplastic fibroblasts in both mammary carcinomas and basal cell carcinomas of the skin. J Clin Pathol 46:429-436
Poulsom R, Pignatelli M, Stetler-Stevenson WG, Liotta LA, Wright PA, Jeffrey RE, Longcroft JM, Rogers L, Stamp GWH (1992) Stromal expression of 72 kDa type IV collagenase (MMP-2) and TIMP-2 mRNAs in colorectal neoplasia. Am J Pathol 141:389-396
Pyke C, Ralfkiaer K, Dano K (1993) Messenger RNA for two type IV collagenases is located in stromal cells in human colon cancer. Am J Pathol 142:359-365
Sato H, Takino T, Okada Y, Cao J, Shinigawa A, Yamamoto E, Seiki M (1994) A matrix metalloproteinase expressed on the surface of invasive tumor cells. Nature 370:61-65
Soini Y, Pääkkö P, Autio-Harmainen H (1993) Genes of laminin B1 chain, a1 (IV) chain of type IV collagen, and 72-kd type IV collagenase are mainly expressed by the stromal cells of lung carcinomas. Am J Pathol 142:1622-1630
Taniguchi S, Iwamura T, Kitamura N (1992) Correlation between spontaneous metastatic potential and type I collagenolytic activity in a human pancreatic cancer cell line (SUIT-2) and sublines. Clin Exp Metastasis 10:259-266
Taniguchi S, Iwamura T, Kiramura N, Yamanari H, Setoguchi T (1994) Heterogeneities of attachment, chemotaxis, and protease production among clones with different metastatic potentials from a human pancreatic cancer cell line. Clin Exp Metastasis 12:238-244
Tryggvason K, Hoyhtya M, Pyke C (1993) Type IV collagenase in invasive tumors. Breast Cancer Res Treat 24:209-218
Urbanski SJ, Edwards DR, Maitland A, Leco KJ, Watson A, Kossakowska AE (1992) Expression of metalloproteinases and their inhibitors in primary pulmonary carcinomas. Br J Cancer 66: 1188-1194
Zucker S, Biswas C (1994) Tumor collagenase-stimulating factor: a paracrine stimulator of fibroblast production of matrix metalloproteinases in cancer. Bull Inst Pasteur 92:284-290
