Association of fibroblastoid features with the invasive phenotype in human bronchial cancer cell lines

[en] The acquisition of a metastatic phenotype by epithelial cells implicates a series of changes altering their differentiation, their overall behavior and morphology. In the present study, we have examined the relationships between the cellular morphology, E-cadherin expression, matrix metalloproteinases expression and in vitro invasive properties in two human bronchial immortalized cell lines. The (16HBE14o-) cell line which did not show any invasive abilities in the Boyden chamber assay displayed a typical epithelial morphology in monolayer, expressed high levels of E-cadherin and synthesized neither MMP-2 and MT1-MMP nor vimentin. In contrast, the BZR cell line which was highly invasive displayed a more elongated phenotype in monolayer, did not produce E-cadherin but expressed vimentin, MMP-2 and MT1-MMP. Our data therefore suggest that the metastatic progression of broncho-pulmonary cancer cells results in a cellular dedifferentiation and the gain of some mesenchymal attributes (loss of E-cadherin and expression of vimentin) associated with enhanced degradative properties (expression of metalloproteinases)

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Polette, M.

Gilles, Christine ; Université de Liège - ULiège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement

de Bentzmann, S.

Gruenert, D.

Tournier, J. M.

Birembaut, P.

Language :

English

Title :

Association of fibroblastoid features with the invasive phenotype in human bronchial cancer cell lines

Publication date :

1998

Journal title :

Clinical and Experimental Metastasis

ISSN :

0262-0898

Publisher :

Kluwer Academic Publishers, Dordrecht, Netherlands

Volume :

Issue :

Pages :

105-12

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 22 June 2010

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