Article (Scientific journals)
The E-cadherin-repressed hNanos1 gene induces tumor cell invasion by upregulating MT1-MMP expression
Bonnomet, A.; Polette, M.; Strumane, K. et al.
2008In Oncogene, 27 (26), p. 3692-9
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Keywords :
hNanos1; E-cadherin; MT1-MMP; cancer; cell invasion
Abstract :
[en] In this study, we examined the role of the E-cadherin-repressed gene human Nanos1 (hNanos1) in tumor invasion process. First, our in vivo study revealed that hNanos1 mRNAs were overexpressed in invasive lung carcinomas. Moreover, hNanos1 was co-localized with MT1-MMP (membrane type 1-matrix metalloproteinase) in E-cadherin-negative invasive lung tumor clusters. Using an inducible Tet-on system, we showed that induction of hNanos1 expression in DLD1 cells increased their migratory and invasive abilities in a three-dimensional migration and in a modified Boyden chamber assay. Accordingly, we demonstrated that hNanos1 upregulated MT1-MMP expression at the mRNA and protein levels. Inversely, using an RNA interference strategy to inhibit hNanos1 expression in invasive Hs578T, BT549 and BZR cancer cells, we observed a downregulation of MT1-MMP mRNA and protein and concomitantly a decrease of the invasive capacities of tumor cells in a modified Boyden chamber assay. Taken together, our results demonstrate that hNanos1, by regulating MT1-MMP expression, plays an important role in the acquisition of invasive properties by epithelial tumor cells.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Bonnomet, A.
Polette, M.
Strumane, K.
Gilles, Christine ;  Université de Liège - ULiège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement
Dalstein, V.
Kileztky, C.
Berx, G.
Van Roy, F.
Birembaut, P.
Nawrocki-Raby, B.
Language :
English
Title :
The E-cadherin-repressed hNanos1 gene induces tumor cell invasion by upregulating MT1-MMP expression
Publication date :
2008
Journal title :
Oncogene
ISSN :
0950-9232
eISSN :
1476-5594
Publisher :
Nature Publishing Group, Basingstoke, United Kingdom
Volume :
27
Issue :
26
Pages :
3692-9
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 22 June 2010

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