Reference : The E-cadherin-repressed hNanos1 gene induces tumor cell invasion by upregulating MT1...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
The E-cadherin-repressed hNanos1 gene induces tumor cell invasion by upregulating MT1-MMP expression
Bonnomet, A. [ > > ]
Polette, M. [ > > ]
Strumane, K. [ > > ]
Gilles, Christine mailto [Université de Liège - ULiège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement >]
Dalstein, V. [ > > ]
Kileztky, C. [ > > ]
Berx, G. [ > > ]
Van Roy, F. [ > > ]
Birembaut, P. [ > > ]
Nawrocki-Raby, B. [ > > ]
Nature Publishing Group
Yes (verified by ORBi)
United Kingdom
[en] hNanos1 ; E-cadherin ; MT1-MMP ; cancer ; cell invasion
[en] In this study, we examined the role of the E-cadherin-repressed gene human Nanos1 (hNanos1) in tumor invasion process. First, our in vivo study revealed that hNanos1 mRNAs were overexpressed in invasive lung carcinomas. Moreover, hNanos1 was co-localized with MT1-MMP (membrane type 1-matrix metalloproteinase) in E-cadherin-negative invasive lung tumor clusters. Using an inducible Tet-on system, we showed that induction of hNanos1 expression in DLD1 cells increased their migratory and invasive abilities in a three-dimensional migration and in a modified Boyden chamber assay. Accordingly, we demonstrated that hNanos1 upregulated MT1-MMP expression at the mRNA and protein levels. Inversely, using an RNA interference strategy to inhibit hNanos1 expression in invasive Hs578T, BT549 and BZR cancer cells, we observed a downregulation of MT1-MMP mRNA and protein and concomitantly a decrease of the invasive capacities of tumor cells in a modified Boyden chamber assay. Taken together, our results demonstrate that hNanos1, by regulating MT1-MMP expression, plays an important role in the acquisition of invasive properties by epithelial tumor cells.

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