The ponA gene of Enterococcus faecalis JH2-2 codes for a low-affinity class a penicillin-binding protein

[en] soluble derivative of the Enterococcus faecalis JH2-2 class A PBP1 (*PBP1) was overproduced and purified. It exhibited a glycosyltransferase activity on the Escherichia coli (14)C(-)labeled lipid 11 precursor. As a DD-peptidase, it could hydrolyze thiolester substrates with efficiencies similar to those of other class A penicillin-binding proteins (PBPs) and bind beta-lactams, but with k(2)/K (a parameter accounting for the acylation step efficiency) values characteristic of penicillin-resistant PBPs.

Disciplines :

Microbiology

Author, co-author :

Duez, Colette ; Université de Liège - ULiège > Centre d'ingénierie des protéines

Hallut, Séverine; Université de Liège - ULiège > CIP

Rhazi, Noureddine ; Université de Liège - ULiège > Centre d'ingénierie des protéines

Amoroso, Ana Maria ; Université de Liège - ULiège > Centre d'ingénierie des protéines

Bouillenne, Fabrice ; Université de Liège - ULiège > Centre d'ingénierie des protéines

Coyette, Jacques ; Université de Liège - ULiège > Services généraux (Faculté des sciences) > Relations académiques et scientifiques (Sciences)

Language :

English

Title :

The ponA gene of Enterococcus faecalis JH2-2 codes for a low-affinity class a penicillin-binding protein

Publication date :

July 2004

Journal title :

Journal of Bacteriology

ISSN :

0021-9193

eISSN :

1098-5530

Publisher :

Amer Soc Microbiology, Washington, United States - Washington

Volume :

186

Issue :

Pages :

4412-4416

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 10 February 2009

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