[en] Asthma is a complex inflammatory disease of airways. A network of reciprocal interactions between inflammatory cells, peptidic mediators, extracellular matrix components, and proteases is thought to be involved in the installation and maintenance of asthma-related airway inflammation and remodeling. To date, new proteic mediators displaying significant activity in the pathophysiology of asthma are still to be unveiled. The main objective of this study was to uncover potential target proteins by using surface-enhanced laser desorption/ionization-time of flight-mass spectrometry (SELDI-TOF-MS) on lung samples from mouse models of allergen-induced airway inflammation and remodeling. In this model, we pointed out several protein or peptide peaks that were preferentially expressed in diseased mice as compared to controls. We report the identification of different five proteins: found inflammatory zone 1 or RELM (FIZZ-1), calcyclin (S100A6), clara cell secretory protein 10 (CC10), Ubiquitin, and Histone H4.
Quesada Calvo, Florence ✱; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques
Fillet, Marianne ✱; Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments
Crahay, Céline ; Université de Liège - ULiège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement
Paulissen, Geneviève ; Université de Liège - ULiège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement
Rocks, Natacha ; Université de Liège - ULiège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement
Guéders, Maud ; Université de Liège - ULiège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement
Wehenkel, Louis ; Université de Liège - ULiège > Dép. d'électric., électron. et informat. (Inst.Montefiore) > Systèmes et modélisation
Merville, Marie-Paule ; Université de Liège - ULiège > Département de pharmacie > Chimie médicale
Louis, Renaud ; Université de Liège - ULiège > Département des sciences cliniques > Pneumologie - Allergologie
Foidart, Jean-Michel ; Université de Liège - ULiège > Département des sciences cliniques > Gynécologie - Obstétrique - Labo de biologie des tumeurs et du développement
Noël, Agnès ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie cellulaire et moléculaire appliquée à l'homme
Cataldo, Didier ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie générales, humaines et path.
✱ These authors have contributed equally to this work.
Language :
English
Title :
Biomarker discovery in asthma-related inflammation and remodeling.
Publication date :
2009
Journal title :
Proteomics
ISSN :
1615-9853
eISSN :
1615-9861
Publisher :
Wiley-VCH Verlag Gmbh, Weinheim, Germany
Volume :
9
Issue :
8
Pages :
2163-2170
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique Walloon region Fondation Léon Fredericq CHU Liège - Centre Hospitalier Universitaire de Liège BELSPO - Belgian Science Policy Office Centre Anticancéreux près de l'Université de Liège BELSPO - Politique scientifique fédérale
Funding text :
This work was supported by grants of the Fonds National de la Recherche Scientifique (FNRS, Brussels, Belgium), the Walloon Region Government (DGTRE project #114/702), the Fondation Leon Fredericq (University of Liàge), the CHU (Liege, Belgium), Action de Recherches Concertées, IAP6/35 network (funded by the Interuniversity Attraction Poles Programme, initiated by the Belgian State, Science Policy Office), the Centre Anticancéreux près de l'Université de Liège, the Interuniversity Attraction Poles Programme- Belgian Science Policy (Brussels, Belgium).
Louis, R., Lau, L.C., Bron, A.O., Roldaan, A.C., The relationship between airways inflammation and asthma severity (2000) Am. J. Respir. Crit. Care. Med, 161, pp. 9-16
Bousquet, J., Chanez, P., Lacoste, J.Y., White, R., Asthma: A disease remodeling the airways (1992) Allergy, 47, pp. 3-11
Cataldo, D.D., Gueders, M.M., Rocks, N., Sounni, N.E., Pathogenic role of matrix metalloproteases and their inhibitors in asthma and chronic obstructive pulmonary disease and therapeutic relevance of matrix metalloproteases inhibitors (2003) Cell. Mol. Biol. (Noisy-le-grand), 49, pp. 875-884
Gueders, M.M., Bertholet, P., Perin, F., Rocks, N., A novel formulation of inhaled doxycycline reduces allergeninduced inflammation, hyperresponsiveness and remodeling by matrix metalloproteinases and cytokines modulation in a mouse model of asthma (2008) Biochem. Pharmacol, 75, pp. 514-526
Lange, P., Parner, J., Vestbo, J., Schnohr, P., Jensen, G., A 15-year follow-up study of ventilatory function in adults with asthma (1998) N. Engl. J. Med, 339, pp. 1194-1200
de Seny, D., Fillet, M., Meuwis, M.A., Geurts, P., Discovery of new rheumatoid arthritis biomarkers using the surface-enhanced laser desorption/ionization time-of-flight mass spectrometry ProteinChip approach (2005) Arthritis Rheum, 52, pp. 3801-3812
de Seny, D., Fillet, M., Ribbens, C., Maree, R., Monomeric Calgranulins Measured by SELDI-TOF Mass Spectrometry and Calprotectin Measured by ELISA as Biomarkers in Arthritis (2008) Clin. Chem, 54, pp. 1066-1075
Kriegova, E., Melle, C., Kolek, V., Hutyrova, B., Protein profiles of bronchoalveolar lavage fluid from patients with pulmonary sarcoidosis (2006) Am. J. Respir. Crit. Care. Med, 173, pp. 1145-1154
Kwapiszewska, G., Meyer, M., Bogumil, R., Bohle, R.M., Identification of proteins in laser-microdissected small cell numbers by SELDI-TOF and Tandem MS (2004) BMC Biotechnol, 4, p. 30
Merkel, D., Rist, W., Seither, P., Weith, A., Lenter, M.C., Proteomic study of human bronchoalveolar lavage fluids from smokers with chronic obstructive pulmonary disease by combining surface-enhanced laser desorption/ionizationmass spectrometry profiling with mass spectrometric protein identification (2005) Proteomics, 5, pp. 2972-2980
Meuwis, M.A., Fillet, M., Geurts, P., de Seny, D., Biomarker discovery for inflammatory bowel disease, using proteomic serum profiling (2007) Biochem. Pharmacol, 73, pp. 1422-1433
Geurts, P., Fillet, M., de Seny, D., Meuwis, M.A., Proteomic mass spectra classification using decision tree based ensemble methods (2005) Bioinformatics, 21, pp. 3138-3145
Kips, J.C., Tournoy, K.G., Pauwels, R.A., Gene knockout models of asthma (2000) Am. J. Respir. Crit. Care. Med, 162, pp. S66-S70
Sakai, K., Yokoyama, A., Kohno, N., Hamada, H., Hiwada, K., Prolonged antigen exposure ameliorates airway inflammation but not remodeling in a mouse model of bronchial asthma (2001) Int. Arch. Allergy Immunol, 126, pp. 126-134
Tournoy, K.G., Hove, C., Grooten, J., Moerloose, K., Animal models of allergen-induced tolerance in asthma: Are T-regulatory-1 cells (Tr-1) the solution for T-helper-2 cells (Th-2) in asthma? (2006) Clin. Exp. Allergy, 36, pp. 8-20
Murphy, V.E., Johnson, R.F., Wang, Y.C., Akinsanya, K., Proteomic study of plasma proteins in pregnant women with asthma (2006) Respirology, 11, pp. 41-48
Wu, J., Kobayashi, M., Sousa, E.A., Liu, W., Differential proteomic analysis of bronchoalveolar lavage fluid in asthmatics following segmental antigen challenge (2005) Mol. Cell Proteomics, 4, pp. 1251-1264
Hall, I.P., Genetics and pulmonary medicine 8: Asthma (1999) Thorax, 54 (1), pp. 55-69
Nair, M.G., Gallagher, I.J., Taylor, M.D., Loke, P., Chitinase and Fizz family members are a generalized feature of nematode infection with selective upregulation of Ym1 and Fizz1 by antigen-presenting cells (2005) Infect. Immun, 73, pp. 385-394
Liu, T., Jin, H., Ullenbruch, M., Hu, B., Regulation of found in inflammatory zone 1 expression in bleomycininduced lung fibrosis: Role of IL-4/IL-13 and mediation via STAT-6 (2004) J. Immunol, 173, pp. 3425-3431
Pesce, J., Kaviratne, M., Ramalingam, T.R., Thompson, R.W., The IL-21 receptor augments Th2 effector function and alternative macrophage activation (2006) J. Clin. Invest, 116, pp. 2044-2055
Davies, D.E., Wicks, J., Powell, R.M., Puddicombe, S.M., Holgate, S.T., Airway remodeling in asthma: New insights (2003) J. Allergy Clin. Immunol, 111, pp. 215-225. , quiz 226
Klug, J., Beier, H.M., Bernard, A., Chilton, B.S., Uteroglobin/Clara cell 10 kDa family of proteins: Nomenclature committee report (2000) Ann. N Y Acad. Sci, 923, pp. 348-354
Lindahl, M., Svartz, J., Tagesson, C., Demonstration of different forms of the anti-inflammatory proteins lipocortin-1 and Clara cell protein-16 in human nasal and bronchoalveolar lavage fluids (1999) Electrophoresis, 20, pp. 881-890
Breen, E.C., Tang, K., Calcyclin (S100A6) regulates pulmonary fibroblast proliferation, morphology, and cytoskeletal organization in vitro (2003) J. Cell. Biochem, 88, pp. 848-854
Barnes, P.J., Adcock, I.M., Ito, K., Histone acetylation and deacetylation: Importance in inflammatory lung diseases (2005) Eur. Respir. J, 25, pp. 552-563
Tomita, K., Barnes, P.J., Adcock, I.M., The effect of oxidative stress on histone acetylation and IL-8 release (2003) Biochem. Biophys. Res. Commun, 301, pp. 572-577
Elliott, P.J., Pien, C.S., McCormack, T.A., Chapman, I.D., Adams, J., Proteasome inhibition: A novel mechanism to combat asthma (1999) J. Allergy Clin. Immunol, 104, pp. 294-300
