[en] Resveratrol is a polyphenolic antioxidant present in beverage and food known for its multiple protective effects. We report the inhibitory effects of resveratrol on equine myeloperoxidase (MPO), a hemic peroxidase present in the granules of the neutrophils involved in the inflammatory response. Resveratrol inhibited the production of reactive oxygen species (ROS) by stimulated equine neutrophils by acting as a direct scavenger of the ROS released by the cells but did not modify the degranulation of the stimulated neutrophils as the amounts of released MPO were unchanged. Resveratrol strongly inhibited the chlorination, oxidation, and nitration activities of MPO in a dose-dependent manner. By an original technique of specific immunological extraction followed by enzymatic detection (SIEFED), we demonstrated that resveratrol inhibited the peroxidasic activity of the MPO measured by a direct interaction such as the fixation of resveratrol on the enzyme. The observation of a decrease of the accumulation of compound II suggested that resveratrol acts as an electron donor for MPO reduction.
Research Center/Unit :
CORD - Centre de l'Oxygène, Recherche et Développement - ULiège
Disciplines :
Chemistry Agriculture & agronomy Food science
Author, co-author :
Kohnen, Stephan ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Franck, Thierry ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Van Antwerpen, Pierre; Université Libre de Bruxelles - ULB > Chimie Pharmaceutique Organique (CPO)
Boudjeltia, Karim Zouaoui; Université Libre de Bruxelles - ULB > Laboratory of Experimental Medicine
Mouithys-Mickalad, Ange ; Université de Liège - ULiège > Centre de l'oxygène : Recherche et développement (C.O.R.D.)
Deby, Christiane ; Centre Hospitalier Universitaire de Liège - CHU > Administration des patients - Admission des hospitalisés
Moguilevsky, Nicole; Facultés Universitaires Notre-Dame de la Paix - Namur - FUNDP > Laboratoire d'Allergologie expérimentale
Deby-Dupont, Ginette; Université de Liège - ULiège > Centre de l'Oxygène, Recherche et développement (C.O.R.D.)
Lamy, Maurice ; Université de Liège - ULiège > Département des sciences cliniques > Anesthésie et réanimation
Serteyn, Didier ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Language :
English
Title :
Resveratrol inhibits the activity of equine neutrophil Myeloperoxidase by a direct interaction with the enzyme
Publication date :
03 October 2007
Journal title :
Journal of Agricultural and Food Chemistry
ISSN :
0021-8561
eISSN :
1520-5118
Publisher :
Amer Chemical Soc, Washington, United States - Washington
Volume :
55
Issue :
20
Pages :
8080-8087
Peer reviewed :
Peer Reviewed verified by ORBi
Name of the research project :
MPOEQUI
Funders :
DGTRE - Région wallonne. Direction générale des Technologies, de la Recherche et de l'Énergie
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