Article (Scientific journals)
Bacterial Intestinal Flora Associated with Enterotoxaemia in Belgian Blue Calves
Manteca, Christophe; Daube, Georges; Pirson, Vincianne et al.
2001In Veterinary Microbiology, 81 (1), p. 21-32
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Keywords :
Enterotoxaemia; Calves; Clostridium perfringens; E. coli; Salmonella
Abstract :
[en] The enterotoxaemia syndrome in Belgian Blue calves is characterised by a high case fatality rate, sudden death, lesions of haemorrhagic enteritis of the small intestine and, quite often an absence of other clinical signs but its cause has not been yet identified. As a first step in this identification, the aerobic and anaerobic intestinal flora of a population of 78 calves, originating from farms located in southern Belgium and that died in circumstances defined as "calf enterotoxaemia" (study population) and of 64 calves that died in other circumstances (control population) were studied qualitatively and quantitatively. The colonies were identified after subcultures with appropriate API sugar sets. Anaerobically Clostridium perfringens was isolated in higher numbers (mean values of 10(7)-10(7.5) colony forming units (CFU) versus 10(4)-10(5) CFU per ml of intestinal content) and from more animals (79 versus 19%) in the study population than in the control population, although individual results from both populations could overlap. Other clostridial species, i.e. mainly urease-negative C. sordellii and C. bifermentans, were isolated in high numbers (>10(6) CFU per ml of intestinal content) from a few animals in the study population only. All but one of the 705 C. perfringens isolates from both populations belonged to the A toxin type and none of the urease-negative C. sordellii was toxigenic. Gram-negative anaerobes were not isolated in high numbers from any of the samples. Aerobically beta-haemolytic E. coli were significantly more frequent among the study population, but were isolated from only 25% of the animals. Salmonella Typhimurium was isolated from only two animals in the study population. Less than 1% of the E. coli isolated were verotoxigenic and one-third were necrotoxigenic. At this stage only non-enterotoxigenic type A C. perfringens are thus statistically associated with the enterotoxaemia syndrome in Belgian Blue calves and fulfil the first of the Koch's postulates.
Disciplines :
Microbiology
Veterinary medicine & animal health
Author, co-author :
Manteca, Christophe;  Université de Liège - ULiège - ULG > Maladies infectieuses et parasitaires > Bactériologie et Pathologies des maladies bactériennes
Daube, Georges  ;  Université de Liège - ULiège > Département de sciences des denrées alimentaires > Microbiologie des denrées alimentaires
Pirson, Vincianne;  Université de Liège - ULiège - ULG > Maladies infectieuses et parasitaires > Bactériologie et Pathologie des Maladies bactériennes
Limbourg, Bernard;  Fédération de Lutte contre les Maladies du bétail de Ciney
Kaeckenbeeck, Albert ;  Université de Liège - ULiège > Relations académiques et scientifiques (Méd. vétérinaire)
Mainil, Jacques ;  Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Bactériologie et pathologie des maladies bactériennes
Language :
English
Title :
Bacterial Intestinal Flora Associated with Enterotoxaemia in Belgian Blue Calves
Publication date :
2001
Journal title :
Veterinary Microbiology
ISSN :
0378-1135
eISSN :
1873-2542
Publisher :
Elsevier, Netherlands
Volume :
81
Issue :
1
Pages :
21-32
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 14 September 2008

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