Virulence Plasmids of Enterotoxigenic Escherichia Coli Isolates from Piglets

[en] Virulence plasmids of 68 ETEC isolates from piglets belonging to different pathotypes and six ETEC isolates from calves with pathotypes typical of porcine ETEC were identified with seven virulence probes for the heat-stable (STa and STb) and heat-labile (LT) enterotoxins, for the F4, F5, F6, and F41 fimbrial adhesion subunit, and also with five Rep probes for the RepFIA and RepFIB basic replicons, and the RepFIC family of basic replicons. With the exception of the F41 probe, the other virulence probes hybridized with at least one plasmid band of a size range from 65 to more than 100 Mda. Common associations of virulence factor-encoding genes on plasmid bands were: STb/LT, STa/F5, STa/F6, STa/STb. Other associations, STa/F4, STa/F4/F6, and STa/STb/LT/F6, were rarer. On the other hand the F4 adhesin-encoding genes were isolated on one plasmid band in all but three F4+ isolates. All but one of the 92 virulence plasmids which were studied have Rep probe hybridization profiles and replicon types typical of the uni- or multireplicon plasmids belonging to the various incompatibility groups of the F incompatibility complex.

Disciplines :

Microbiology

Author, co-author :

Mainil, Jacques ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Bactériologie et pathologie des maladies bactériennes

Daube, Georges ; Université de Liège - ULiège > Département de sciences des denrées alimentaires > Microbiologie des denrées alimentaires

Jacquemin, Etienne; Université de Liège - ULiège - ULG > Maladies infectieuses et parasitaires > Bactériologie et Pathologie des Maladies Bactériennes

Pohl, Pohl; INRV

Kaeckenbeeck, Albert ; Université de Liège - ULiège > Maladies infectieuses et parasitaires > Bactériologie et Pathologie des Maladies Bactériennes

Language :

English

Title :

Virulence Plasmids of Enterotoxigenic Escherichia Coli Isolates from Piglets

Publication date :

1998

Journal title :

Veterinary Microbiology

ISSN :

0378-1135

eISSN :

1873-2542

Publisher :

Elsevier, Netherlands

Volume :

Issue :

Pages :

291-301

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 14 September 2008

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Bibliography

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