Article (Scientific journals)
Insights into nuclear organization in plants as revealed by the dynamic distribution of Arabidopsis SR splicing factors
Tillemans, Vinciane; Leponce, Isabelle; Rausin, Glwadys et al.
2006In Plant Cell, 18 (11), p. 3218-3234
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Keywords :
SR proteins; splicing; Arabidopsis thaliana
Abstract :
[en] Serine/arginine-rich (SR) proteins are splicing regulators that share a modular structure consisting of one or two N-terminal RNA recognition motif domains and a C-terminal RS-rich domain. We investigated the dynamic localization of the Arabidopsis thaliana SR protein RSZp22, which, as we showed previously, distributes in predominant speckle-like structures and in the nucleolus. To determine the role of RSZp22 diverse domains in its nucleolar distribution, we investigated the subnuclear localization of domain-deleted mutant proteins. Our results suggest that the nucleolar localization of RSZp22 does not depend on a single targeting signal but likely involves different domains/motifs. Photobleaching experiments demonstrated the unrestricted dynamics of RSZp22 between nuclear compartments. Selective inhibitor experiments of ongoing cellular phosphorylation influenced the rates of exchange of RSZp22 between the different nuclear territories, indicating that SR protein mobility is dependent on the phosphorylation state of the cell. Furthermore, based on a leptomycin B- and fluorescence loss in photobleaching-based sensitive assay, we suggest that RSZp22 is a nucleocytoplasmic shuttling protein. Finally, with electron microscopy, we confirmed that RSp31, a plant-specific SR protein, is dynamically distributed in nucleolar cap-like structures upon phosphorylation inhibition. Our findings emphasize the high mobility of Arabidopsis SR splicing factors and provide insights into the dynamic relationships between the different nuclear compartments.
Disciplines :
Biochemistry, biophysics & molecular biology
Phytobiology (plant sciences, forestry, mycology...)
Author, co-author :
Tillemans, Vinciane ;  Université de Liège - ULiège > Département des sciences de la vie > Génomique fonctionnelle et imagerie moléculaire végétale
Leponce, Isabelle ;  Université de Liège - ULiège > Département des sciences de la vie > Génomique fonctionnelle et imagerie moléculaire végétale
Rausin, Glwadys ;  Université de Liège - ULiège > Département des sciences de la vie > Génomique fonctionnelle et imagerie moléculaire végétale
Dispa, Laurence 
Motte, Patrick ;  Université de Liège - ULiège > Département des sciences de la vie > Génomique fonctionnelle et imagerie moléculaire végétale
Language :
English
Title :
Insights into nuclear organization in plants as revealed by the dynamic distribution of Arabidopsis SR splicing factors
Publication date :
November 2006
Journal title :
Plant Cell
ISSN :
1040-4651
eISSN :
1532-298X
Publisher :
Amer Soc Plant Biologists, Rockville, United States - Maryland
Volume :
18
Issue :
11
Pages :
3218-3234
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 30 January 2009

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