[en] Bifidobacteria are normal intestinal flora in humans and animals. The genus Bifidobacterium includes 31 species of significant host specificity. Taking into account their properties, we proposed to use bifidobacteria as fecal contamination indicators. PCR-restriction fragment length polymorphism on the 16S rDNA gene was used to distinguish the different Bifidobacterium species. Sixty-four strains belonging to 13 different species were differentiated from animal or human origin using one or two restriction enzymes. Moreover, the primers used were specifics of the Bifidobacterium genus. Therefore, this method made it possible to determine both the presence of bifidobacteria in a sample and its origin of contamination.
Disciplines :
Microbiology Food science
Author, co-author :
Delcenserie, Véronique ; Université de Liège - ULiège > Département de sciences des denrées alimentaires > Gestion de la qualité dans la chaîne alimentaire
Bechoux, Nathalie ; Université de Liège - ULiège > Département de sciences des denrées alimentaires > Microbiologie des denrées alimentaires
Leonard, Tamara; Université de Liège - ULiège > Sciences des Denrées alimentaires > Microbiologie des Denrées alimentaires
China, Bernard; Université de Liège - ULiège > Sciences des Denrées alimentaires > Microbiologie des Denrées alimentaires
Daube, Georges ; Université de Liège - ULiège > Département de sciences des denrées alimentaires > Microbiologie des denrées alimentaires
Language :
English
Title :
Discrimination between Bifidobacterium species from human and animal origin by PCR-restriction fragment length polymorphism
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