Molecular recognition; sensors; Atomic Force Microscopy (AFM)
Abstract :
[en] With the purpose of developing biosensors, the reliable proof of the biological activity of two new sensor systems was obtained by atomic force microscopy (AFM) in both the imaging and the single-molecule force spectroscopy modes. Antigens or antibodies of pharmacological interest were grafted onto self-assembled monolayers of thiols on gold, and AFM imaging demonstrated that the grafting process produced homogeneous submonolayers of isolated proteins. The analysis of the morphology of the surfaces at the different functionalization steps allowed evaluating the protein grafting density and showed that the recognition of complementary species present in the surrounding solution occurred. Single-molecule force spectroscopy experiments between the sensing surfaces and AFM probes, onto which the complementary species were grafted, enabled a direct and rapid test of the biological activity of the sensors by investigating the interaction occurring at the level of one single ligand-receptor bond. Ellipsometry and surface plasmon resonance allowed further characterization of the sensor surfaces and confirmed that the biological recognition took place.
Disciplines :
Chemistry Materials science & engineering
Author, co-author :
Cecchet, Francesca; Université catholique de Louvain (UCL) > Unité de chimie et de physique des hauts polymères (POLY) and Research Center on Micro- and Nanoscopic Materials and Electronic Devices (CeRMiN)
Duwez, Anne-Sophie ; Université catholique de Louvain (UCL) > Present address: University of Liège, Department of Chemistry, NanoChemistry and Molecular Systems
Gabriel, Sabine ; Université de Liège - ULiège > Department of Chemistry > Center for Education and Research on Macromolecules (CERM)
Jérôme, Christine ; Université de Liège - ULiège > Department of Chemistry > Center for Education and Research on Macromolecules (CERM)
Jérôme, Robert ; Université de Liège - ULiège > Department of Chemistry > Center for Education and Research on Macromolecules (CERM)
Glinel, Karine; Université catholique de Louvain (UCL) > Unité de chimie et de physique des hauts polymères (POLY) and Research Center on Micro- and Nanoscopic Materials and Electronic Devices (CeRMiN)
Demoustier-Champagne, Sophie; Université catholique de Louvain (UCL) > Unité de chimie et de physique des hauts polymères (POLY) and Research Center on Micro- and Nanoscopic Materials and Electronic Devices (CeRMiN)
Jonas, Alain M.; Université catholique de Louvain (UCL) > Unité de chimie et de physique des hauts polymères (POLY) and Research Center on Micro- and Nanoscopic Materials and Electronic Devices (CeRMiN)
Nysten, Bernard; Université catholique de Louvain (UCL) > Unité de chimie et de physique des hauts polymères (POLY) and Research Center on Micro- and Nanoscopic Materials and Electronic Devices (CeRMiN)
Language :
English
Title :
Atomic force microscopy investigation of the morphology and the biological activity of protein-modified surfaces for bio- and immunosensors
Publication date :
01 September 2007
Journal title :
Analytical Chemistry
ISSN :
0003-2700
eISSN :
1520-6882
Publisher :
Amer Chemical Soc, Washington, United States - Washington
Région wallonne : Direction générale des Technologies, de la Recherche et de l'Energie - DGTRE, (research convention 01/1/5016 “NanoSens”) F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE] BELSPO - SPP Politique scientifique - Service Public Fédéral de Programmation Politique scientifique
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