Cdk2 is critical for proliferation and self-renewal of neural progenitor cells in the adult subventricular zone

[en] We investigated the function of cyclin-dependent kinase 2 (Cdk2) in neural progenitor cells during postnatal development. Chondroitin sulfate proteoglycan (NG2)-expressing progenitor cells of the subventricular zone (SVZ) show no significant difference in density and proliferation between Cdk2(-/-) and wild-type mice at perinatal ages and are reduced only in adult Cdk2(-/-) mice. Adult Cdk2(-/-) SVZ cells in culture display decreased self-renewal capacity and enhanced differentiation. Compensatory mechanisms in perinatal Cdk2(-/-) SVZ cells, which persist until postnatal day 15, involve increased Cdk4 expression that results in retinoblastoma protein inactivation. A subsequent decline in Cdk4 activity to wild-type levels in postnatal day 28 Cdk2(-/-) cells coincides with lower NG2(+) proliferation and self-renewal capacity similar to adult levels. Cdk4 silencing in perinatal Cdk2(-/-) SVZ cells abolishes Cdk4 up-regulation and reduces cell proliferation and self-renewal to adult levels. Conversely, Cdk4 overexpression in adult SVZ cells restores proliferative capacity to wildtype levels. Thus, although Cdk2 is functionally redundant in perinatal SVZ, it is important for adult progenitor cell proliferation and self-renewal through age-dependent regulation of Cdk4.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Jablonska, Beata

Aguirre, Adan A.

Vandenbosch, Renaud ; Université de Liège - ULiège > CNCM/ Centre fac. de rech. en neurobiologie cell. et moléc.

Belachew, Shibeshih ; Université de Liège - ULiège > Département des sciences cliniques > Neurologie

Berthet, Cyril

Kaldis, Philipp

Gallo, Vittorio

Language :

English

Title :

Cdk2 is critical for proliferation and self-renewal of neural progenitor cells in the adult subventricular zone

Publication date :

17 December 2007

Journal title :

Journal of Cell Biology

ISSN :

0021-9525

eISSN :

1540-8140

Publisher :

Rockefeller Univ Press, New York, United States - New York

Volume :

179

Issue :

Pages :

1231-1245

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 28 January 2009

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