Monomeric calgranulins measured by SELDI-TOF mass spectrometry and calprotectin measured by ELISA as biomarkers in arthritis

De Seny, Dominique; Fillet, Marianne; Ribbens, Clio; Marée, Raphaël; Meuwis, Marie-Alice; Lutteri, Laurence; Chapelle, Jean-Paul; Wehenkel, Louis; Louis, Edouard; Merville, Marie-Paule; Malaise, Michel

doi:10.1373/clinchem.2007.099549

Article (Scientific journals)

Monomeric calgranulins measured by SELDI-TOF mass spectrometry and calprotectin measured by ELISA as biomarkers in arthritis

De Seny, Dominique; Fillet, Marianne; Ribbens, Clio et al.

2008 • In Clinical Chemistry, 54, p. 1066-1075

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/4957

DOI
10.1373/clinchem.2007.099549

PubMed
18436720

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Keywords :

Biological Markers; Calgranulin A; Calgranulin B; Leukocyte L1 Antigen Complex; S100 Proteins; Serum Amyloid A Protein; Arthritis, Rheumatoid; Enzyme-Linked Immunosorbent Assay; Psoriasis

Abstract :

[en] BACKGROUND: SELDI-TOF mass spectrometry (MS) is a high-throughput proteomic approach with potential for identifying novel forms of serum biomarkers of arthritis. METHODS: We used SELDI-TOF MS to analyze serum samples from patients with various forms of inflammatory arthritis. Several protein profiles were collected on different Bio-Rad Laboratories ProteinChip arrays (CM10 and IMAC-Cu(2+)) and were evaluated statistically to select potential biomarkers. RESULTS: SELDI-TOF MS analyses identified several calgranulin proteins [S100A8 (calgranulin A), S100A9 (calgranulin B), S100A9*, and S100A12 (calgranulin C)], serum amyloid A (SAA), SAA des-Arg (SAA-R), and SAA des-Arg/des-Ser (SAA-RS) as biomarkers and confirmed the results with other techniques, such as western blotting, immunoprecipitation, and nano-LC-MS/MS. The S100 proteins were all able to significantly differentiate samples from patients with rheumatoid arthritis (RA), psoriatic arthritis (PsA), and ankylosing spondylitis (AS) from those of patients with inflammatory bowel diseases used as an inflammatory control (IC) group, whereas the SAA, SAA-R, and SAA-RS proteins were not, with the exception of AS. The 4 S100 proteins were coproduced in all of the pathologies and were significantly correlated with the plasma calprotectin concentration; however, these S100 proteins were correlated with the SAA peak intensities only in the RA and IC patient groups. In RA, these S100 proteins (except for S100A12) were significantly correlated with the serum concentrations of C-reactive protein, matrix metalloproteinase 3, and anti-cyclic citrullinated peptide and with the Disease Activity Score (DAS(28)). CONCLUSIONS: The SELDI-TOF MS technology is a powerful approach for analyzing the status of monomeric, truncated, or posttranslationally modified forms of arthritis biomarkers, such as the S100A8, S100A9, S100A12, and SAA proteins. The fact that the SELDI-TOF MS data were correlated with results obtained with the classic calprotectin ELISA test supports the reliability of this new proteomic technique.

Disciplines :

Gastroenterology & hepatology
Rheumatology

Author, co-author :

De Seny, Dominique ; Centre Hospitalier Universitaire de Liège - CHU > Rhumatologie

Fillet, Marianne ; Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments

Ribbens, Clio ; Centre Hospitalier Universitaire de Liège - CHU > Rhumatologie

Marée, Raphaël ; Université de Liège - ULiège > GIGA-Management : Plateforme bioinformatique

Meuwis, Marie-Alice ; Université de Liège - ULiège > GIGA-Management : Plate-forme protéomique

Lutteri, Laurence ; Centre Hospitalier Universitaire de Liège - CHU > Chimie médicale

Chapelle, Jean-Paul ; Centre Hospitalier Universitaire de Liège - CHU > Chimie médicale

Wehenkel, Louis ; Université de Liège - ULiège > Dép. d'électric., électron. et informat. (Inst.Montefiore) > Systèmes et modélisation

Louis, Edouard ; Université de Liège - ULiège > Département des sciences cliniques > Hépato-gastroentérologie - Relations académiques et scientifiques (Médecine)

Merville, Marie-Paule ; Université de Liège - ULiège > Département de pharmacie > Chimie médicale

Malaise, Michel ; Centre Hospitalier Universitaire de Liège - CHU > Rhumatologie

Language :

English

Title :

Monomeric calgranulins measured by SELDI-TOF mass spectrometry and calprotectin measured by ELISA as biomarkers in arthritis

Publication date :

2008

Journal title :

Clinical Chemistry

ISSN :

0009-9147

eISSN :

1530-8561

Publisher :

American Association for Clinical Chemistry, Washington, United States - District of Columbia

Volume :

Pages :

1066-1075

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 28 January 2009

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