Identification of factors that maintain mammalian outer hair cells in adult organ of Corti explants

[en] Both outer hair cells (OHCs) and inner hair cells (IHCs) survive and mature in 3 days old rat organ of Corti explants cultured for I month in a minimal essential medium. In contrast. under the same culture conditions, only IHCs survive in explants from adult guinea pig organ of Corti while many of the OHCs are lost within the first 48 It. Hair cell Count,, show OHCs loss to be greater in the lower portion (i.e. middle turn) of the cochlea than Lit the apex. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling (TUNEL) indicates that there is DNA damage in adult OHCs, within 8 h of explantation. Treatment of the adult organ of Corti explants with either actinomycin D (10(-7) M) or cycloheximide (10(-6) M) prevents most OHC losses . According to these results apoptosis may be the mechanism of OHC loss in adult organ of Corti explants, Stable membrane potentials recorded from the OHCs in both uncultured and actinomycin D-treated organ of Corti explants cultured for 72 h demonstrate the functional integrity of these hair cells. OHC losses in the adult guinea pig, organ of Corti cultures can also be prevented by treatment with several of the growth factors tested. i.e. acidic fibroblast growth factor (aFGF), insulin-like growth factor-1 (IGF-1), epidermal growth factor (EGF), transforming growth factor-beta1 (TGF-beta1). and glial cell-derived neurotrophic factor (GDNF). The results of this study suggest that growth factor therapy may be applicable to the treatment of some hearing disorders. (C) 2002 Elsevier Science B.V. All rights reserved.

Disciplines :

Otolaryngology
Anatomy (cytology, histology, embryology...) & physiology

Author, co-author :

Malgrange, Brigitte ; Université de Liège - ULiège > CNCM/ Centre fac. de rech. en neurobiologie cell. et moléc.

Rigo, Jean-Michel; Universiteit Hasselt - UH

Coucke, Paul; Université de Liège - ULiège > Département des sciences de la vie > Biologie cellulaire

Thiry, Marc ; Centre Hospitalier Universitaire de Liège - CHU > Anesthésie et réanimation

Hans, Grégory ; Université de Liège - ULiège > Département des sciences cliniques > Neurologie

Nguyen, Laurent

van de Water, Thomas R.; Université de Liège - ULiège > Département des sciences cliniques > Neurologie - Doyen de la Faculté de Médecine

Moonen, Gustave ; Centre Hospitalier Universitaire de Liège - CHU > O.R.L.

Lefebvre, Philippe

Language :

English

Title :

Identification of factors that maintain mammalian outer hair cells in adult organ of Corti explants

Publication date :

August 2002

Journal title :

Hearing Research

ISSN :

0378-5955

eISSN :

1878-5891

Publisher :

Elsevier Science Bv, Amsterdam, Netherlands

Volume :

170

Issue :

1-2

Pages :

48-58

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 28 January 2009

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