Glycine Triggers an Intracellular Calcium Influx in Oligodendrocyte Progenitor Cells Which Is Mediated by the Activation of Both the Ionotropic Glycine Receptor and Na+-Dependent Transporters
[en] Using fluo-3 calcium imaging, we demonstrate that glycine induces an increase in intracellular calcium concentration ([Ca2+]i) in cortical oligodendrocyte progenitor (OP) cells. This effect results from a calcium entry through voltage-gated calcium channels (VGCC), as it is observed only in OP cells expressing such channels, and it is abolished either by removal of calcium from the extracellular medium or by application of an L-type VGCC blocker. Glycine-triggered Ca2+ influx in OP cells actually results from an initial depolarization that is the consequence of the activation of both the ionotropic glycine receptor (GlyR) and Na+-dependent transporters, most probably the glycine transporters 1 (GLYT1) and/or 2 (GLYT2) which are colocalized in these cells. Through this GlyR- and transporter-mediated effect on OP intrcellular calcium concentration [Ca2+]i, glycine released by neurons may, as well as other neurotransmitters, serve as a signal between neurons and OP during development.
Belachew, Shibeshih ; Université de Liège - ULiège > Département des sciences cliniques > Neurologie
Malgrange, Brigitte ; Université de Liège - ULiège > CNCM/ Centre fac. de rech. en neurobiologie cell. et moléc.
Rigo, Jean-Michel; Universiteit Hasselt - UH
Rogister, Bernard ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie générales, et biochimie humaine
Leprince, Pierre ; Université de Liège - ULiège > CNCM/ Centre fac. de rech. en neurobiologie cell. et moléc.
Hans, Grégory ; Centre Hospitalier Universitaire de Liège - CHU > Anesthésie et réanimation
Nguyen, Laurent ; Université de Liège - ULiège > Département des sciences cliniques > Neurologie
Moonen, Gustave ; Université de Liège - ULiège > Département des sciences cliniques > Neurologie - Doyen de la Faculté de Médecine
Language :
English
Title :
Glycine Triggers an Intracellular Calcium Influx in Oligodendrocyte Progenitor Cells Which Is Mediated by the Activation of Both the Ionotropic Glycine Receptor and Na+-Dependent Transporters
Publication date :
June 2000
Journal title :
European Journal of Neuroscience
ISSN :
0953-816X
eISSN :
1460-9568
Publisher :
Blackwell, Oxford, United Kingdom
Volume :
12
Issue :
6
Pages :
1924-30
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
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