Article (Scientific journals)
Rapid, postmortem 9.4 T MRI of spinal cord injury: correlation with histology and survival times.
Scholtes, Félix; Phan-Ba, Rémy; Theunissen, Evi et al.
2008In Journal of Neuroscience Methods, 174 (2), p. 157-67
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Keywords :
Animals; Female; Image Processing, Computer-Assisted; Magnetic Resonance Imaging; Rats; Rats, Wistar; Spinal Cord Injuries/pathology; Time
Abstract :
[en] High field magnetic resonance imaging (MRI) has been increasingly used to assess experimental spinal cord injury (SCI). In the present investigation, after partial spinal cord injury and excision of the whole spine, pathological changes of the spinal cord were studied in spinal cord-spine blocks, from the acute to the chronic state (24 h to 5 months). Using proton density (PD) weighted imaging parameters at a magnetic field strength of 9.4 tesla (T), acquisition times ranging from <1 to 10 h per specimen were used. High in-plane pixel resolution (68 and 38 microm, respectively) was obtained, as well as high signal-to-noise ratio (SNR), which is important for optimal contrast settings. The quality of the resulting MR images was demonstrated by comparison with histology. The cord and the lesion were shown in their anatomical surroundings, detecting cord swelling in the acute phase (24 h to 1 week) and cord atrophy at the chronic stage. Haemorrhage was detected as hypo-intense signal. Oedema, necrosis and scarring were hyper-intense but could not be distinguished. Histology confirmed that the anatomical delimitation of the lesion extent by MRI was precise, both with high and moderate resolution. The present investigation thus demonstrates the precision of spinal cord MRI at different survival delays after compressive partial SCI and establishes efficient imaging parameters for postmortem PD MRI.
Disciplines :
Anatomy (cytology, histology, embryology...) & physiology
Author, co-author :
Scholtes, Félix  ;  Centre Hospitalier Universitaire de Liège - CHU > Neurochirurgie
Phan-Ba, Rémy ;  Université de Liège - ULiège > Département des sciences cliniques > Département des sciences cliniques
Theunissen, Evi
Adriaensens, Peter
Brook, Gary
Franzen, Rachelle  ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Neuro-anatomie
Bouhy, Delphine
Gelan, Jan
Martin, Didier  ;  Centre Hospitalier Universitaire de Liège - CHU > Neurochirurgie
Schoenen, Jean  ;  Centre Hospitalier Universitaire de Liège - CHU > Neurologie CHR
Language :
English
Title :
Rapid, postmortem 9.4 T MRI of spinal cord injury: correlation with histology and survival times.
Publication date :
2008
Journal title :
Journal of Neuroscience Methods
ISSN :
0165-0270
Publisher :
Elsevier Science, Amsterdam, Netherlands
Volume :
174
Issue :
2
Pages :
157-67
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 26 January 2009

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