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Synthesis of [Gamma-32p]Thiamine Triphosphate
Grandfils, Christian; Bettendorff, Lucien; de Rycker, C. et al.
1988In Analytical Biochemistry, 169 (2), p. 274-278
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Abstract :
[en] We developed a novel chemical synthesis of thiamine triphosphate which allows us to incorporate 32P in the gamma position. The reaction is based on the condensation of [32P]orthophosphoric acid and thiamine diphosphate in the presence of ethyl chloroformate. After purification by two ion-exchange purification steps, the thiamine derivative has a specific radioactivity of 10 Ci/mmol. The average final yield synthesis is about 10%.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Grandfils, Christian ;  Université de Liège - ULiège > Biochimie et physiologie générale
Bettendorff, Lucien  ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie humaine et pathologique
de Rycker, C.
Schoffeniels, Ernest
Language :
English
Title :
Synthesis of [Gamma-32p]Thiamine Triphosphate
Publication date :
1988
Journal title :
Analytical Biochemistry
ISSN :
0003-2697
eISSN :
1096-0309
Publisher :
Elsevier, Atlanta, United States - Florida
Volume :
169
Issue :
2
Pages :
274-278
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 23 January 2009

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